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The Production of Recombinant African Swine Fever Virus Lv17/WB/Rie1 Strains and Their In Vitro and In Vivo Characterizations.
Petrini, Stefano; Righi, Cecilia; Mészáros, István; D'Errico, Federica; Tamás, Vivien; Pela, Michela; Olasz, Ferenc; Gallardo, Carmina; Fernandez-Pinero, Jovita; Göltl, Eszter; Magyar, Tibor; Feliziani, Francesco; Zádori, Zoltán.
Afiliación
  • Petrini S; National Reference Centre for Pestiviruses and Asfivirus, Istituto Zooprofilattico Sperimentale Umbria-Marche "Togo Rosati", Via Gaetano Salvemini, 1, 06126 Perugia, Italy.
  • Righi C; National Reference Centre for Pestiviruses and Asfivirus, Istituto Zooprofilattico Sperimentale Umbria-Marche "Togo Rosati", Via Gaetano Salvemini, 1, 06126 Perugia, Italy.
  • Mészáros I; HUN-REN Veterinary Medical Research Institute (VMRI), Hungária krt. 21, 1143 Budapest, Hungary.
  • D'Errico F; National Reference Centre for Pestiviruses and Asfivirus, Istituto Zooprofilattico Sperimentale Umbria-Marche "Togo Rosati", Via Gaetano Salvemini, 1, 06126 Perugia, Italy.
  • Tamás V; HUN-REN Veterinary Medical Research Institute (VMRI), Hungária krt. 21, 1143 Budapest, Hungary.
  • Pela M; National Reference Centre for Pestiviruses and Asfivirus, Istituto Zooprofilattico Sperimentale Umbria-Marche "Togo Rosati", Via Gaetano Salvemini, 1, 06126 Perugia, Italy.
  • Olasz F; HUN-REN Veterinary Medical Research Institute (VMRI), Hungária krt. 21, 1143 Budapest, Hungary.
  • Gallardo C; European Union Reference Laboratory for ASF (EURL-ASF), Centro de Investigación en Sanidad Animal (CISA-INIA, CSIC), Valdeolmos, 28130 Madrid, Spain.
  • Fernandez-Pinero J; European Union Reference Laboratory for ASF (EURL-ASF), Centro de Investigación en Sanidad Animal (CISA-INIA, CSIC), Valdeolmos, 28130 Madrid, Spain.
  • Göltl E; HUN-REN Veterinary Medical Research Institute (VMRI), Hungária krt. 21, 1143 Budapest, Hungary.
  • Magyar T; HUN-REN Veterinary Medical Research Institute (VMRI), Hungária krt. 21, 1143 Budapest, Hungary.
  • Feliziani F; National Reference Centre for Pestiviruses and Asfivirus, Istituto Zooprofilattico Sperimentale Umbria-Marche "Togo Rosati", Via Gaetano Salvemini, 1, 06126 Perugia, Italy.
  • Zádori Z; HUN-REN Veterinary Medical Research Institute (VMRI), Hungária krt. 21, 1143 Budapest, Hungary.
Vaccines (Basel) ; 11(12)2023 Dec 17.
Article en En | MEDLINE | ID: mdl-38140263
ABSTRACT
Lv17/WB/Rie1-Δ24 was produced via illegitimate recombination mediated by low-dilution serial passage in the Cos7 cell line and isolated on PAM cell culture. The virus contains a huge ~26.4 Kb deletion in the left end of its genome. Lv17/WB/Rie1-ΔCD-ΔGL was generated via homologous recombination, crossing two ASFV strains (Lv17/WB/Rie1-ΔCD and Lv17/WB/Rie1-ΔGL containing eGFP and mCherry markers) during PAM co-infection. The presence of unique parental markers in the Lv17/WB/Rie1-ΔCD-ΔGL genome indicates at least two recombination events during the crossing, suggesting that homologous recombination is a relatively frequent event in the ASFV genome during replication in PAM. Pigs infected with Lv17/WB/Rie1-Δ24 and Lv17/WB/Rie1/ΔCD-ΔGL strains have shown mild clinical signs despite that ASFV could not be detected in their sera until a challenge infection with the Armenia/07 ASFV strain. The two viruses were not able to induce protective immunity in pigs against a virulent Armenia/07 challenge.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vaccines (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vaccines (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza