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BML-111, the agonist of lipoxin A4, suppresses epithelial-mesenchymal transition and migration of MCF-7 cells via regulating the lipoxygenase pathway.
Xu, Fen; Zhou, Xiaoyan; Lin, Lan; Xu, Jing; Feng, Yu; He, Yuanqiao; Hao, Hua.
Afiliación
  • Xu F; Department of General Medicine, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, China.
  • Zhou X; Department of Pathophysiology, Medical College of Nanchang University, Nanchang, China.
  • Lin L; Department of Pathology, Second Affiliated Hospital of Nanchang University, Nanchang, China.
  • Xu J; Department of Pathology, Second Affiliated Hospital of Nanchang University, Nanchang, China.
  • Feng Y; Department of Pathology, Second Affiliated Hospital of Nanchang University, Nanchang, China.
  • He Y; Department of Laboratory Animal Science, Medical College of Nanchang University, Nanchang, China.
  • Hao H; Department of Pathology, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, China.
Int J Immunopathol Pharmacol ; 37: 3946320231223826, 2023.
Article en En | MEDLINE | ID: mdl-38134963
ABSTRACT

Introduction:

Aberrant epithelial-mesenchymal transition (EMT) and migration frequently occur during tumour progression. BML-111, an analogue of lipoxin A4, has been implicated in inflammation in cancer research.

Methods:

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, western blot, Reverse Transcription Polymerase Chain Reaction (RT-PCR), transwell assay, immunofluorescence, and immunohistochemistry were conducted in this study.

Results:

In vitro experiments revealed that BML-111 inhibited EMT and migration in CoCl2-stimulated MCF-7 cells. These effects were achieved by inhibiting MMP-2 and MMP-9, which are downregulated by 5-lipoxygenase (5-LOX). Moreover, BML-111 inhibited EMT and migration of breast cancer cells in BALB/c nude mice inoculated with MCF-7 cells.

Conclusion:

Our results suggest that BML-111 may be a potential therapeutic drug for breast cancer and that blocking the 5-LOX pathway could be a possible approach for mining effective drug targets.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Lipoxinas Límite: Animals / Female / Humans Idioma: En Revista: Int J Immunopathol Pharmacol Asunto de la revista: ALERGIA E IMUNOLOGIA / FARMACOLOGIA / PATOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Lipoxinas Límite: Animals / Female / Humans Idioma: En Revista: Int J Immunopathol Pharmacol Asunto de la revista: ALERGIA E IMUNOLOGIA / FARMACOLOGIA / PATOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido