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Effect of follicular wave synchronization using a progesterone-releasing intravaginal device-based protocol on in vitro embryo production in Bos taurus cows subjected to 14-day intervals ovum pick-up.
Anchordoquy, Juan Patricio; Balbi, Marianela; Farnetano, Nicolás A; Giuliodori, Mauricio J; Nikoloff, Noelia; Carranza-Martín, Ana C; Furnus, Cecilia C; Anchordoquy, Juan Mateo.
Afiliación
  • Anchordoquy JP; IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argentina; Cátedra de Fisiología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argent
  • Balbi M; IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argentina.
  • Farnetano NA; IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argentina.
  • Giuliodori MJ; Cátedra de Fisiología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argentina; INIRA - Instituto de Investigaciones en Reproducción Animal (UNLP-CONICET La Plata), Facultad de Ciencias Veterinarias-Universidad Nacional de La Plata, Buenos Aires, Argentina. Elect
  • Nikoloff N; IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argentina.
  • Carranza-Martín AC; IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argentina.
  • Furnus CC; IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argentina.
  • Anchordoquy JM; IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argentina; Cátedra de Fisiología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argent
Anim Reprod Sci ; 261: 107397, 2024 Feb.
Article en En | MEDLINE | ID: mdl-38134500
ABSTRACT
Follicular wave synchronization (FWS) before ovum pick-up (OPU) is one of the strategies used to improve the efficiency of in vitro embryo production (IVP). This study aimed to evaluate the effect of FWS on the total follicular number, cumulus-oocyte complex (COC) recovery, and in vitro embryo development in Angus cows (n = 33) subjected to OPU with 14-day intersession intervals. Additionally, it was also evaluated the presence of carryover effects given the short intersession interval used. The experiment was run as a 2-treatment (FWS vs. Control) x 2-period (1 vs. 2) crossover design. Animals in the FWS group received an intravaginal progesterone implant (1gr), estradiol benzoate (2 mg), and D-cloprostenol (150 µg) on day 0 and the OPU was performed on day 5. Control group animals did not receive any hormone treatment. The FWS increased the number of 6-10 mm follicles (P = 0.05), but it decreased the COC recovery rate (P < 0.01). The FWS did not affect the total or frozen embryo numbers (P = 0.49 and P = 0.17; respectively), but it increased the total blastocyst cell number (P < 0.01). A carryover effect was found on the total and < 6 mm follicles number (P = 0.10 and P < 0.01; respectively), and on the regular, atretic, viable, and total number of COC (P = 0.01, P = 0.08, P = 0.02 and P < 0.01; respectively). We concluded that the FWS increased the quality of embryos after OPU with 14-day intersession intervals in Angus cows and that this kind of OPU/IVP scheme enabled the existence of a carryover effect, especially on the follicle number and COC morphology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Progesterona / Recuperación del Oocito Límite: Animals Idioma: En Revista: Anim Reprod Sci Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Progesterona / Recuperación del Oocito Límite: Animals Idioma: En Revista: Anim Reprod Sci Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos