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A smartphone-based electrochemical POCT for CEA based on signal amplification of Zr6MOFs.
Shi, Shan-Shan; Li, Xiao-Jian; Ma, Rong-Na; Shang, Lei; Zhang, Wei; Zhao, Huai-Qing; Jia, Li-Ping; Wang, Huai-Sheng.
Afiliación
  • Shi SS; School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong, 250022, P. R. China. chm_zhaohq@ujn.edu.cn.
  • Li XJ; College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, P. R. China. jialiping@lcu.edu.cn.
  • Ma RN; College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, P. R. China. jialiping@lcu.edu.cn.
  • Shang L; College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, P. R. China. jialiping@lcu.edu.cn.
  • Zhang W; College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, P. R. China. jialiping@lcu.edu.cn.
  • Zhao HQ; College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, P. R. China. jialiping@lcu.edu.cn.
  • Jia LP; School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong, 250022, P. R. China. chm_zhaohq@ujn.edu.cn.
  • Wang HS; College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, P. R. China. jialiping@lcu.edu.cn.
Lab Chip ; 24(2): 367-374, 2024 01 17.
Article en En | MEDLINE | ID: mdl-38126214
ABSTRACT
Carcinoembryonic antigen (CEA) is a biomarker of high expression in cancer cells. Highly sensitive and selective detection of CEA holds significant clinical value in the diagnosis, monitoring and efficacy evaluation of malignant tumors. In this work, a smartphone-based electrochemical point-of-care testing (POCT) platform for the detection of CEA was developed based on a Zr6MOF signal amplification strategy. Ferrocene labeled DNA strands (Fc-DNA) were immobilized on Zr6MOFs to form a Fc-DNA/Zr6MOF signal probe. Double-stranded DNA (dsDNA) formed by complementary DNA (cDNA) and CEA aptamer was assembled on a screen-printed electrode via an Au-S bond. When CEA was added, the aptamer specifically bound with CEA, resulting in the exposure of cDNA. Then, Fc-DNA/Zr6MOF signal probes were introduced on the electrode surface through hybridization between Fc-DNA and cDNA. The detection of CEA was realized by measuring the electrochemical response of Fc. The POCT device was made by connecting a modified electrode with a smartphone through a Sensit Smart USB flash disk. Due to the signal amplification of Zr6MOFs, this POCT platform exhibited high sensitivity, wide linear range, and low detection limit for CEA detection. The developed POCT platform has been used for the detection of CEA in actual human serum samples with satisfactory results.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Aptámeros de Nucleótidos Límite: Humans Idioma: En Revista: Lab Chip Asunto de la revista: BIOTECNOLOGIA / QUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Aptámeros de Nucleótidos Límite: Humans Idioma: En Revista: Lab Chip Asunto de la revista: BIOTECNOLOGIA / QUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido