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Exploring the potential of the halotolerant bacterial strain Bacillus subtilis LN8B as an ecofriendly sulfide collector for seawater flotation.
Arias, Dayana; Saldaña, Manuel; Botero, Yesica L; Dinamarca, Francisco; Paredes, Bernardo; Salazar-Ardiles, Camila; Andrade, David C; Cisternas, Luis A; Carrasco, Jorge; Santos, Carlos; Dorador, Cristina; Gómez-Silva, Benito.
Afiliación
  • Arias D; Laboratory of Molecular Biology and Applied Microbiology, Research Center in High Altitude Medicine and Physiology, Biomedical Department, Faculty of Health Science, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile.
  • Saldaña M; Faculty of Engineering and Architecture, Arturo Prat University, Iquique 1110939, Chile.
  • Botero YL; Departamento de Ingeniería Química y Procesos de Minerales, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile.
  • Dinamarca F; Biochemistry Lab., Biomedical Dept., Health Sciences Faculty and Centre for Biotechnology and Bioengineering (CeBiB), Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile.
  • Paredes B; Biochemistry Lab., Biomedical Dept., Health Sciences Faculty and Centre for Biotechnology and Bioengineering (CeBiB), Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile.
  • Salazar-Ardiles C; Laboratory of Molecular Biology and Applied Microbiology, Research Center in High Altitude Medicine and Physiology, Biomedical Department, Faculty of Health Science, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile.
  • Andrade DC; Exercise Applied Physiology Laboratory, Research Center in High Altitude Medicine and Physiology, Biomedical Department, Faculty of Health Science, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile.
  • Cisternas LA; Departamento de Ingeniería Química y Procesos de Minerales, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile.
  • Carrasco J; Departamento de Ingeniería en Minas, Facultad de Ingeniería, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile.
  • Santos C; Departamento de Ingeniería en Minas, Facultad de Ingeniería, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile.
  • Dorador C; Department of Biotechnology, Faculty of Marine Sciences and Biological Resources and Centre for Biotechnology and Bioengineering (CeBiB), Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile.
  • Gómez-Silva B; Biochemistry Lab., Biomedical Dept., Health Sciences Faculty and Centre for Biotechnology and Bioengineering (CeBiB), Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile.
J Appl Microbiol ; 135(1)2024 Jan 02.
Article en En | MEDLINE | ID: mdl-38126104
ABSTRACT

AIM:

To assess the effectiveness of Bacillus subtilis strain LN8B as a biocollector for recovering pyrite (Py) and chalcopyrite (CPy) in both seawater (Sw) and deionized water (Dw), and to explore the underlying adhesion mechanism in these bioflotation experiments. MATERIALS AND

METHODS:

The bioflotation test utilized B. subtilis strain LN8B as the biocollector through microflotation experiments. Additionally, frother methyl isobutyl carbinol (MIBC) and conventional collector potassium amyl xanthate (PAX) were introduced in some experiments. The zeta potential (ZP) and Fourier-transform infrared spectroscopy (FTIR) was employed to explore the adhesion mechanism of Py and CPy interacting with the biocollector in Sw and Dw. The adaptability of the B. subtilis strain to different water types and salinities was assessed through growth curves measuring optical density. Finally, antibiotic susceptibility tests were conducted to evaluate potential risks of the biocollector.

RESULTS:

Superior outcomes were observed in Sw where Py and CPy recovery was ∼39.3% ± 7.7% and 41.1% ± 5.8%, respectively, without microorganisms' presence. However, B. subtilis LN8B potentiate Py and CPy recovery, reaching 72.8% ± 4.9% and 84.6% ± 1.5%, respectively. When MIBC was added, only the Py recovery was improved (89.4% ± 3.6%), depicting an adverse effect for CPy (81.8% ± 1.1%). ZP measurements indicated increased mineral surface hydrophobicity when Py and CPy interacted with the biocollector in both Sw and Dw. FTIR revealed the presence of protein-related amide peaks, highlighting the hydrophobic nature of the bacterium. The adaptability of this strain to diverse water types and salinities was assessed, demonstrating remarkable growth versatility. Antibiotic susceptibility tests indicated that B. subtilis LN8B was susceptible to 23 of the 25 antibiotics examined, suggesting it poses minimal environmental risks.

CONCLUSIONS:

The study substantiates the biotechnological promise of B. subtilis strain LN8B as an efficient sulfide collector for promoting cleaner mineral production. This effectiveness is attributed to its ability to induce mineral surface hydrophobicity, a result of the distinct characteristics of proteins within its cell wall.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus subtilis / Cobre / Hierro / Minerales Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus subtilis / Cobre / Hierro / Minerales Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Reino Unido