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Genomic characterization of a unique Panton-Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities.
Ramsay, Joshua P; Parahitiyawa, Nipuna; Mowlaboccus, Shakeel; Mullally, Christopher A; Yee, Nicholas W T; Shoby, Princy; Colombi, Elena; Tan, Hui-Leen; Pearson, Julie C; Coombs, Geoffrey W.
Afiliación
  • Ramsay JP; Curtin Medical School, Faculty of Health Sciences, Curtin University, Perth, WA, Australia.
  • Parahitiyawa N; Curtin Health Innovation Research Institute, Faculty of Health Sciences, Curtin University, Perth, WA, Australia.
  • Mowlaboccus S; Curtin Medical School, Faculty of Health Sciences, Curtin University, Perth, WA, Australia.
  • Mullally CA; Microbiology Department, Fiona Stanley Hospital, PathWest Laboratory Medicine, Murdoch, WA, Australia.
  • Yee NWT; Antimicrobial Resistance and Infectious Disease (AMRID) Research Laboratory, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia.
  • Shoby P; Antimicrobial Resistance and Infectious Disease (AMRID) Research Laboratory, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia.
  • Colombi E; Antimicrobial Resistance and Infectious Disease (AMRID) Research Laboratory, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia.
  • Tan HL; Antimicrobial Resistance and Infectious Disease (AMRID) Research Laboratory, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia.
  • Pearson JC; Curtin Medical School, Faculty of Health Sciences, Curtin University, Perth, WA, Australia.
  • Coombs GW; Curtin Health Innovation Research Institute, Faculty of Health Sciences, Curtin University, Perth, WA, Australia.
Microb Genom ; 9(12)2023 Dec.
Article en En | MEDLINE | ID: mdl-38117559
ABSTRACT
In 2010 a single isolate of a trimethoprim-resistant multilocus sequence type 5, Panton-Valentine leucocidin-positive, community-associated methicillin-resistant Staphylococcus aureus (PVL-positive ST5 CA-MRSA), colloquially named WA121, was identified in northern Western Australia (WA). WA121 now accounts for ~14 % of all WA MRSA infections. To gain an understanding of the genetic composition and phylogenomic structure of WA121 isolates we sequenced the genomes of 155 WA121 isolates collected 2010-2021 and present a detailed genomic description. WA121 was revealed to be a single clonally expanding lineage clearly distinct from sequenced ST5 strains reported outside Australia. WA121 strains were typified by the presence of the distinct PVL phage φSa2wa-st5, the recently described methicillin resistance element SCCmecIVo carrying the trimethoprim resistance (dfrG) transposon Tn4791, the novel ß-lactamase transposon Tn7702 and the epidermal cell differentiation inhibitor (EDIN-A) plasmid p2010-15611-2. We present evidence that SCCmecIVo together with Tn4791 has horizontally transferred to Staphylococcus argenteus and evidence of intragenomic movement of both Tn4791 and Tn7702. We experimentally demonstrate that p2010-15611-2 is capable of horizontal transfer by conjugative mobilization from one of several WA121 isolates also harbouring a pWBG749-like conjugative plasmid. In summary, WA121 is a distinct and clonally expanding Australian PVL-positive CA-MRSA lineage that is increasingly responsible for infections in indigenous communities in northern and western Australia. WA121 harbours a unique complement of mobile genetic elements and is capable of transferring antimicrobial resistance and virulence determinants to other staphylococci.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus Resistente a Meticilina País/Región como asunto: Oceania Idioma: En Revista: Microb Genom Año: 2023 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus Resistente a Meticilina País/Región como asunto: Oceania Idioma: En Revista: Microb Genom Año: 2023 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido