Your browser doesn't support javascript.
loading
Mimicking Nonribosomal Peptides from the Marine Actinomycete Streptomyces sp. H-KF8 Leads to Antimicrobial Peptides.
Beyer, Luisa I; Schäfer, Ann-Britt; Undabarrena, Agustina; Mattsby-Baltzer, Inger; Tietze, Daniel; Svensson, Elin; Stubelius, Alexandra; Wenzel, Michaela; Cámara, Beatriz; Tietze, Alesia A.
Afiliación
  • Beyer LI; Department of Chemistry and Molecular Biology, Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Medicinaregatan 7B, Gothenburg 413 90, Sweden.
  • Schäfer AB; Center for Antibiotic Resistance Research in Gothenburg, University of Gothenburg, Box 100, Göteborg 405 30, Sweden.
  • Undabarrena A; Department of Life Sciences, Chalmers University of Technology, Kemigården 4, Göteborg 412 96, Sweden.
  • Mattsby-Baltzer I; Center for Antibiotic Resistance Research in Gothenburg, University of Gothenburg, Box 100, Göteborg 405 30, Sweden.
  • Tietze D; Departamento de Química & Centro de Biotecnología Daniel Alkalay Lowitt, Laboratorio de Microbiología Molecular y Biotecnología Ambiental, Universidad Técnica Federico Santa María, Valparaíso 2340000, Chile.
  • Svensson E; Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy at University of Gothenburg, University of Gothenburg, Box 440, Göteborg 405 30, Sweden.
  • Stubelius A; Department of Chemistry and Molecular Biology, Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Medicinaregatan 7B, Gothenburg 413 90, Sweden.
  • Wenzel M; Center for Antibiotic Resistance Research in Gothenburg, University of Gothenburg, Box 100, Göteborg 405 30, Sweden.
  • Cámara B; Department of Life Sciences, Chalmers University of Technology, Kemigården 4, Göteborg 412 96, Sweden.
  • Tietze AA; Department of Life Sciences, Chalmers University of Technology, Kemigården 4, Göteborg 412 96, Sweden.
ACS Infect Dis ; 10(1): 79-92, 2024 01 12.
Article en En | MEDLINE | ID: mdl-38113038
ABSTRACT
Microorganisms within the marine environment have been shown to be very effective sources of naturally produced antimicrobial peptides (AMPs). Several nonribosomal peptides were identified based on genome mining predictions of Streptomyces sp. H-KF8, a marine Actinomycetota isolated from a remote Northern Chilean Patagonian fjord. Based on these predictions, a series of eight peptides, including cyclic peptides, were designed and chemically synthesized. Six of these peptides showed antimicrobial activity. Mode of action studies suggest that two of these peptides potentially act on the cell membrane via a novel mechanism allowing the passage of small ions, resulting in the dissipation of the membrane potential. This study shows that though structurally similar peptides, determined by NMR spectroscopy, the incorporation of small sequence mutations results in a dramatic influence on their bioactivity including mode of action. The qualified hit sequence can serve as a basis for more potent AMPs in future studies.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / Actinobacteria Idioma: En Revista: ACS Infect Dis Año: 2024 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / Actinobacteria Idioma: En Revista: ACS Infect Dis Año: 2024 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos