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Requirement of GrgA for Chlamydia infectious progeny production, optimal growth, and efficient plasmid maintenance.
Lu, Bin; Wang, Yuxuan; Wurihan, Wurihan; Cheng, Andrew; Yeung, Sydney; Fondell, Joseph D; Lai, Zhao; Wan, Danny; Wu, Xiang; Li, Wei Vivian; Fan, Huizhou.
Afiliación
  • Lu B; Department of Parasitology, Central South University Xiangya Medical School, Changsha, Hunan, China.
  • Wang Y; Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
  • Wurihan W; Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
  • Cheng A; Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
  • Yeung S; Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
  • Fondell JD; Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
  • Lai Z; Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
  • Wan D; Greehey Children's Cancer Research Institute, University of Texas Health San Antonio, San Antonio, Texas, USA.
  • Wu X; Department of Molecular Medicine, University of Texas Health San Antonio, San Antonio, Texas, USA.
  • Li WV; Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
  • Fan H; Department of Parasitology, Central South University Xiangya Medical School, Changsha, Hunan, China.
mBio ; 15(1): e0203623, 2024 Jan 16.
Article en En | MEDLINE | ID: mdl-38112466
ABSTRACT
IMPORTANCE Hallmarks of the developmental cycle of the obligate intracellular pathogenic bacterium Chlamydia are the primary differentiation of the infectious elementary body (EB) into the proliferative reticulate body (RB) and the secondary differentiation of RBs back into EBs. The mechanisms regulating these transitions remain unclear. In this report, we developed an effective novel strategy termed dependence on plasmid-mediated expression (DOPE) that allows for the knockdown of essential genes in Chlamydia. We demonstrate that GrgA, a Chlamydia-specific transcription factor, is essential for the secondary differentiation and optimal growth of RBs. We also show that GrgA, a chromosome-encoded regulatory protein, controls the maintenance of the chlamydial virulence plasmid. Transcriptomic analysis further indicates that GrgA functions as a critical regulator of all three sigma factors that recognize different promoter sets at developmental stages. The DOPE strategy outlined here should provide a valuable tool for future studies examining chlamydial growth, development, and pathogenicity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Chlamydia / Chlamydia trachomatis Límite: Humans Idioma: En Revista: MBio Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Chlamydia / Chlamydia trachomatis Límite: Humans Idioma: En Revista: MBio Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos