Your browser doesn't support javascript.
loading
Automatic MDSPE Combined with DART-HRMS for the Rapid Quantitation of 21 Synthetic Cathinones in Urine.
Li, Yawen; Lian, Ru; Sheng, Zhenhai; Mao, Jinting; Mao, Chen; Liang, Chen; Zhang, Peng; Ni, Chunfang; Wang, Rong; Zhang, Yurong.
Afiliación
  • Li Y; China State Institute of Pharmaceutical Industry, State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai 200040, China.
  • Lian R; Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Science, Shanghai 200083, China.
  • Sheng Z; Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Science, Shanghai 200083, China.
  • Mao J; Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Science, Shanghai 200083, China.
  • Mao C; Huangpu Branch of Shanghai Public Security Bureau, Shanghai 200010, China.
  • Liang C; Huangpu Branch of Shanghai Public Security Bureau, Shanghai 200010, China.
  • Zhang P; Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Science, Shanghai 200083, China.
  • Ni C; Shanghai Shaanxi Coal Hi-tech Research Institute Co., Ltd., Shanghai 201613, China.
  • Wang R; Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Science, Shanghai 200083, China.
  • Zhang Y; Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Institute of Forensic Science, Shanghai 200083, China.
J Am Soc Mass Spectrom ; 35(1): 40-49, 2024 Jan 03.
Article en En | MEDLINE | ID: mdl-38109269
ABSTRACT
A new, rapid, and automated method for the quantitation of 21 synthetic cathinones in urine was established using magnetic dispersive solid-phase extraction (MDSPE) in combination with direct analysis in real time-high-resolution mass spectrometry (DART-HRMS). Sample preparation and quantitation were verified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methcathinone-D3, α-PVP-D8, and proadifen (SKF525A) were used as internal standards. Magnetic HLB extractant and NaH2PO4/NaOH buffer (0.2 M, pH 7) were used in automatic MDSPE. All 21 synthetic cathinones could be detected and analyzed by DART-HRMS in under 1 min. It was proven that the linearities of 21 synthetic cathinones were suitable (R2 > 0.99) in the concentration ranges of 0.5-100 ng/mL or 1-100 ng/mL. The precision and accuracy values were all within ±15%, and the samples were stable under various conditions. The average time of each sample from preprocessing to completion of detection was approximately 2 min, allowing for rapid sample analysis. The relative error (RE) of the concentrations obtained by DART-HRMS and LC-MS/MS were within ±13.61%, and the linear coefficient (R) was 0.9964. The results of DART-HRMS and LC-MS/MS provided equivalent values at the 95% confidence level. In summary, a simple, fast, and convenient quantitation method via DART-HRMS was established. This application can be utilized to reduce backlogs and promote rapid case processing.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas en Tándem / Cathinona Sintética Idioma: En Revista: J Am Soc Mass Spectrom Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas en Tándem / Cathinona Sintética Idioma: En Revista: J Am Soc Mass Spectrom Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos