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FolE gene expression for folic acid productivity from optimized and characterized probiotic Lactobacillus delbrueckii.
Khedr, Mohamed; Youssef, Fady Sayed; El-Kattan, Noura; Abozahra, Mahmoud S; Selim, Mohammed N; Yousef, Abdullah; Khalil, Kamal M A; Mekky, Alsayed E.
Afiliación
  • Khedr M; Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr, 11884, Cairo, Egypt. Mohamedkhedr.221@Azhar.edu.eg.
  • Youssef FS; Department of Pharmacology Faculty of Veterinary Medicine, Cairo University, Giza, 1221, Egypt.
  • El-Kattan N; Department of Microbiology, Research Institute of Medical Entomology, General Organization for Teaching Hospitals and Institutes, Giza, Egypt.
  • Abozahra MS; Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr, 11884, Cairo, Egypt.
  • Selim MN; Department of Chemistry and Biochemistry, Florida Atlantic University, Boca Raton, FL, 33433, USA.
  • Yousef A; Microbial Chemistry Department, National Research Centre, 33 El-Buhouth Street, Dokki, 12622, Cairo, Egypt.
  • Khalil KMA; Basic & Medical Sciences Department, Faculty of Dentistry, Alryada University for Science & Technology, Al ryada, Egypt.
  • Mekky AE; Genetic Engineering and Biotechnology Division, Genetics and Cytology Department, National Research Centre, 33 El-Buhouth Street, Dokki, 12622, Cairo, Egypt.
J Genet Eng Biotechnol ; 21(1): 169, 2023 Dec 18.
Article en En | MEDLINE | ID: mdl-38108957
ABSTRACT

BACKGROUND:

Lactobacillus delbrueckii was one of the most common milk lactic acid bacterial strains (LAB) which characterized as probiotic with many health influencing properties.

RESULTS:

Among seven isolates, KH1 isolate was the best producer of folic acid with 100 µg/ml after 48 h of incubation; FolE gene expression after 24 h of incubation was in the highest value in case of KH1 with three folds. Lactose was the best carbon source for this KH1, besides the best next isolates KH80 and KH98. The selected three LAB isolates were identified through 16S rDNA as Lactobacillus delbrueckii. These three isolates have high tolerance against acidic pH 2-3; they give 45, 10, and 22 CFUs at pH 3, besides 9, 6, and 4 CFUs at pH2, respectively. They also have resistance against elevated bile salt range 0.1-0.4%. KH1 recorded 99% scavenging against 97.3% 1000 µg/ml ascorbic acid. Docking study exhibits the binding mode of folic acid which exhibited an energy binding of - 8.65 kcal/mol against DHFR. Folic acid formed four Pi-alkyl, Pi-Pi, and Pi-sigma interactions with Ala9, Ile7, Phe34, and Ile60. Additionally, folic acid interacted with Glu30 and Asn64 by three hydrogen bonds with 1.77, 1.76, and 1.96 Å.

CONCLUSION:

LAB isolates have probiotic properties, antioxidant activity, and desired organic natural source for folic acid supplementation that improve hemoglobin that indicated by docking study interaction.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Genet Eng Biotechnol Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Genet Eng Biotechnol Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Países Bajos