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Vascular Reconstruction with the Cuff Technique in Mouse Orthotopic Liver Transplantation.
Tanaka, Kosuke; Uchida, Yoichiro; Kageyama, Shoichi; Nakamura, Kojiro; Hirao, Hirofumi; Kadono, Kentaro; Kawamoto, Hiroshi; Saga, Kenichi; Kidoguchi, Yuki; Watanabe, Takeshi; Hatano, Etsuro.
Afiliación
  • Tanaka K; Department of Surgery, Graduate School of Medicine, Kyoto University; kosuket0814@kuhp.kyoto-u.ac.jp.
  • Uchida Y; Department of Surgery, Graduate School of Medicine, Kyoto University.
  • Kageyama S; Department of Surgery, Graduate School of Medicine, Kyoto University.
  • Nakamura K; Department of Surgery, Graduate School of Medicine, Kyoto University.
  • Hirao H; Department of Surgery, Graduate School of Medicine, Kyoto University.
  • Kadono K; Department of Surgery, Graduate School of Medicine, Kyoto University.
  • Kawamoto H; Department of Surgery, Graduate School of Medicine, Kyoto University.
  • Saga K; Department of Surgery, Graduate School of Medicine, Kyoto University.
  • Kidoguchi Y; Department of Surgery, Graduate School of Medicine, Kyoto University.
  • Watanabe T; Institute for Frontier Life and Medical Sciences, Kyoto University.
  • Hatano E; Department of Surgery, Graduate School of Medicine, Kyoto University.
J Vis Exp ; (202)2023 Dec 01.
Article en En | MEDLINE | ID: mdl-38108403
ABSTRACT
Mouse orthotopic liver transplantation is an effective methodology for investigating the underlying mechanisms of liver ischemia and reperfusion injury. However, the technical challenges pose a barrier to utilizing this valuable experimental model and passing on these skills to the next generation. The most challenging aspect of this procedure is vascular reconstruction, including the portal vein (PV), infrahepatic inferior vena cava (IHIVC), and suprahepatic inferior vena cava. The use of plastic cuffs, rather than sutures, allows for smoother PV and IHIVC reconstruction. Vessels are reconstructed by attaching a cuff made from an intravenous catheter to the tip of the graft vessel and interposing the cuff into the recipient vessel. The two most crucial aspects are properly visualizing the inner lumen of the vessel and avoiding the use of excessive force. Our aim is to provide a technical overview of vascular reconstructions using the cuff technique in recipient surgery. These technical tips for the cuff technique are expected to help microsurgeons facilitate vascular reconstruction and advance their research.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión / Trasplante de Hígado Límite: Animals Idioma: En Revista: J Vis Exp Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión / Trasplante de Hígado Límite: Animals Idioma: En Revista: J Vis Exp Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos