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Decoy peptides effectively inhibit the binding of SARS-CoV-2 to ACE2 on oral epithelial cells.
Loi, Lai-Keng; Yang, Cheng-Chieh; Lin, Yu-Cheng; Su, Yee-Fun; Juan, Yi-Chen; Chen, Yi-Hsin; Chang, Hsiu-Chuan.
Afiliación
  • Loi LK; Department of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan.
  • Yang CC; Department of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan.
  • Lin YC; Department of Stomatology, Oral & Maxillofacial Surgery, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Su YF; Department of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan.
  • Juan YC; iStat Biomedical Co., Ltd, New Taipei City, Taiwan.
  • Chen YH; iStat Biomedical Co., Ltd, New Taipei City, Taiwan.
  • Chang HC; Institute of Oral Biology, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Heliyon ; 9(12): e22614, 2023 Dec.
Article en En | MEDLINE | ID: mdl-38107325
ABSTRACT
The entry of SARS-CoV-2 into host cells involves the interaction between the viral spike protein and the human angiotensin-converting enzyme 2 (ACE2) receptor. Given that the spike protein evolves rapidly to evade host immunity, therapeutics that block ACE2 accessibility, such as spike decoys, could serve as an alternative strategy for attenuating viral infection. Here, we constructed a drug screening platform based on oral epithelial cells to rapidly identify peptides or compounds capable of blocking the spike-ACE2 interaction. We engineered short decoy peptides, 8 to 14 amino acids in length, using the spike protein's receptor-binding motif (RBM) and demonstrated that these peptides can effectively inhibit virus attachment to host cells. Additionally, we discovered that diminazene aceturate (DIZE), an ACE2 activator, similarly inhibited virus binding. Our research thus validates the potential of decoy peptides as a new therapeutic strategy against SARS-CoV-2 infections, opening avenues for further development and study.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2023 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2023 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido