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Co-expression of GR79 EPSPS and GAT generates high glyphosate-resistant alfalfa with low glyphosate residues.
Meng, Yingying; Zhang, Wenwen; Wang, Zhaoming; Yuan, Feng; Guo, Sandui; Lin, Hao; Niu, Lifang.
Afiliación
  • Meng Y; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
  • Zhang W; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
  • Wang Z; Inner Mongolia Pratacultural Technology Innovation Center Co., Ltd, Hohhot, 010010 China.
  • Yuan F; National Center of Pratacultural Technology Innovation (Under Preparation), Hohhot, 010010 China.
  • Guo S; Inner Mongolia Pratacultural Technology Innovation Center Co., Ltd, Hohhot, 010010 China.
  • Lin H; National Center of Pratacultural Technology Innovation (Under Preparation), Hohhot, 010010 China.
  • Niu L; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
aBIOTECH ; 4(4): 352-358, 2023 Dec.
Article en En | MEDLINE | ID: mdl-38106433
ABSTRACT
Weed competition seriously threatens the yield of alfalfa, the most important forage legume worldwide, thus generating herbicide-resistant alfalfa varieties is becoming a necessary cost-effective strategy to assist farmers for weed control. Here, we report the co-expression of plant codon-optimized forms of GR79 EPSPS (pGR79 EPSPS) and N-acetyltransferase (pGAT) genes, in alfalfa, via Agrobacterium-mediated transformation. We established that the pGR79 EPSPS-pGAT co-expression alfalfa lines were able to tolerate up to tenfold higher commercial usage of glyphosate and produced approximately ten times lower glyphosate residues than the conventional cultivar. Our findings generate an elite herbicide-resistant germplasm for alfalfa breeding and provide a promising strategy for developing high-glyphosate-resistant and low-glyphosate-residue forages. Supplementary Information The online version contains supplementary material available at 10.1007/s42994-023-00119-3.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ABIOTECH Año: 2023 Tipo del documento: Article Pais de publicación: Singapur
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ABIOTECH Año: 2023 Tipo del documento: Article Pais de publicación: Singapur