Your browser doesn't support javascript.
loading
Analysis of vitamin D3-sulfate and 25-hydroxyvitamin D3-sulfate in breastmilk by LC-MS/MS.
Reynolds, Carmen J; Dyer, Roy B; Vizenor, Brady A; Koszewski, Nicholas J; Singh, Ravinder J; Thacher, Tom D.
Afiliación
  • Reynolds CJ; Mayo Clinic Department of Physiology & Biomedical Engineering, 200 First St. SW, Rochester, MN 55905 USA. Electronic address: Reynolds.Carmen@mayo.edu.
  • Dyer RB; Mayo Clinic Department of Laboratory Medicine & Pathology, 200 First St. SW, Rochester, MN 55905 USA.
  • Vizenor BA; Mayo Clinic Department of Laboratory Medicine & Pathology, 200 First St. SW, Rochester, MN 55905 USA.
  • Koszewski NJ; GlycoMyr Inc, 3359 Stagecoach Rd, Ames, IA 50010 USA.
  • Singh RJ; Mayo Clinic Department of Laboratory Medicine & Pathology, 200 First St. SW, Rochester, MN 55905 USA.
  • Thacher TD; Mayo Clinic Department of Family Medicine, 200 First St. SW, Rochester, MN 55905 USA.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1232: 123954, 2024 Jan 01.
Article en En | MEDLINE | ID: mdl-38101284
ABSTRACT
Sulfated metabolites of vitamin D have been suggested to be in breastmilk, although current methods to measure sulfated vitamin D compounds in breastmilk by liquid chromatography-tandem mass spectrometry (LC-MS/MS) have not adequately accounted for increased aqueous solubility of these sulfated metabolites. The purpose of this study was to generate a method of LC-MS/MS for measuring vitamin D3-3-sulfate (VitD3-S) and 25-hydroxyvitamin D3-3-sulfate (25OHD3-S) specifically in human breastmilk. The resulting method uses methanol to precipitate protein and solid phase extraction to prepare the samples for LC-MS/MS. The limits of quantification for analytes in solvent were 0.23 ng/mL VitD3-S and 0.2 ng/mL 25OHD3-S. Various experiments observed concentrations ranging 0.53 to 1.7 ng/mL VitD3-S and ≤ 0.29 ng/mL 25OHD3-S. Both analytes were present in aqueous skim milk, demonstrating the enhanced aqueous solubility of these vitamin D sulfates. In conclusion, we describe an effective method for measuring VitD3-S and 25OHD3-S in breastmilk by LC-MS/MS.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcifediol / Espectrometría de Masas en Tándem Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcifediol / Espectrometría de Masas en Tándem Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos