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A rapid and efficient method using electroporation for releasing intracellular microcystin toxins from cultured and naturally occurring cyanobacterial cells in lake water.
Ai, Yuehan; Bertani, Paul; Yang, Hao; Lee, Seungjun; Lu, Wu; Lee, Jiyoung.
Afiliación
  • Ai Y; Department of Food Science and Technology, The Ohio State University, Columbus, OH 43210, USA.
  • Bertani P; Department of Electrical and Computer Engineering, The Ohio State University, Columbus, OH 43210, USA.
  • Yang H; Department of Electrical and Computer Engineering, The Ohio State University, Columbus, OH 43210, USA.
  • Lee S; Department of Food Science and Nutrition, Pukyong National University, Busan, Republic of Korea.
  • Lu W; Department of Electrical and Computer Engineering, The Ohio State University, Columbus, OH 43210, USA.
  • Lee J; Department of Food Science and Technology, The Ohio State University, Columbus, OH 43210, USA; Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, OH 43210, USA. Electronic address: lee.3598@osu.edu.
Mar Pollut Bull ; 198: 115890, 2024 Jan.
Article en En | MEDLINE | ID: mdl-38101057
ABSTRACT
In cyanotoxin measurements, effective release of intracellular cyanotoxins through cell lysis is pivotal. The conventional method for cell lysis is repeated freeze-thaw (F-T), which has several disadvantages, including poor reproducibility since it is operator and equipment dependency and time-consuming. In this study, a rapid and sensitive method was developed using irreversible electroporation, reducing quantification time by over 6 h compared to F-T. Focusing on microcystins (MCs), we developed the most optimal electroporation medium (50 mM Tris (pH 7.0) with 0.5 % SDS) and determined the optimal intensity of electroporation using Microcystis culture. Microcystis cell rupture was validated by scanning electron microscopy. COMSOL simulations mirrored experimental conditions. Compared to F-T, this new method generated an average 13.7 % (6.7 ppb) more MCs from lake water samples (p ≥ 0.05). This innovation, surpassing the time-consuming F-T process, emerges as a valuable tool for timely decision-making in water safety advisory and cyanotoxin management in various settings.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cianobacterias / Microcystis Idioma: En Revista: Mar Pollut Bull Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cianobacterias / Microcystis Idioma: En Revista: Mar Pollut Bull Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido