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A target-triggered ultra-sensitive aptasensor for simultaneous detection of Cd2+ and Hg2+ using MWCNTs-Au NPs modified electrode.
Pan, Yi; Wang, Li; Chen, Shouhui; Wei, Yang; Wei, Xinlin.
Afiliación
  • Pan Y; Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
  • Wang L; Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
  • Chen S; Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
  • Wei Y; Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
  • Wei X; Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China. Electronic address: foodlab2010@163.com.
Food Chem ; 440: 138185, 2024 May 15.
Article en En | MEDLINE | ID: mdl-38100966
ABSTRACT
A sensitive electrochemical assay for simultaneously detecting cadmium ion (Cd2+) and mercury ion (Hg2+) with the aptamer as recognition unit was established, in which methylene blue (MB) and target-triggered in-situ generated Ag nanoclusters (Ag NCs) were identified as signal reporters. Multi-walled carbon nanotubes and gold nanoparticles composites were prepared with polyethyleneimine to amplify electrical signals of screen-printed electrodes. Due to the particular base sequences, MB labeled Cd2+ aptamer paired with ssDNA through T-Hg-T structure with Hg2+. Notably, the C-rich structure in ssDNA acted as a template for the generation of Ag NCs, which could induce differential pulse voltammetry signals corresponding to Hg2+ concentrations. This electrochemical aptasensor exhibited detection limits of 94.01 pg/mL and 15.74 pg/mL for Cd2+ and Hg2+, respectively. The developed aptasensor allowed for practical application to tea and vegetable samples with satisfactory accuracy. This work possesses potential in developing biosensing technologies for simultaneous determination of multiple heavy metals.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Nanotubos de Carbono / Aptámeros de Nucleótidos / Nanopartículas del Metal / Mercurio Idioma: En Revista: Food Chem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Nanotubos de Carbono / Aptámeros de Nucleótidos / Nanopartículas del Metal / Mercurio Idioma: En Revista: Food Chem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido