Your browser doesn't support javascript.
loading
The generation and validation of two NKX2-5 fluorescent reporter human embryonic stem cell lines: UMANe002-A-1 and UMANe002-A-2.
Douglas, M; O'Loughlin, C; Lynch, A T; Prialgauskaite, R; Adamson, A D; Dibb, K M; Kimber, S J; Birket, M J.
Afiliación
  • Douglas M; The University of Manchester, UK.
  • O'Loughlin C; The University of Manchester, UK.
  • Lynch AT; The University of Manchester, UK.
  • Prialgauskaite R; The University of Manchester, UK.
  • Adamson AD; The University of Manchester, UK.
  • Dibb KM; The University of Manchester, UK.
  • Kimber SJ; The University of Manchester, UK.
  • Birket MJ; The University of Manchester, UK. Electronic address: matthew.birket@manchester.ac.uk.
Stem Cell Res ; 74: 103262, 2024 02.
Article en En | MEDLINE | ID: mdl-38100908
ABSTRACT
The transcription factor NKX2-5 is a highly conserved master regulator of heart development which is widely expressed in cardiac progenitors and cardiomyocytes. Fluorescent reporters of NKX2-5 that minimally perturb normal protein expression can enable the identification, quantification and isolation of NKX2-5-expressing cells in a normal physiological state. Here we report the generation of two new hESC lines with eGFP inserted upstream (5') or downstream (3') of NKX2-5, linked by a cleavable T2A peptide. These complementary reporters produce a robust fluorescent signal in cardiac cells and have wide utility particularly for research on developmental biology and disease modelling.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Embrionarias Humanas Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Embrionarias Humanas Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Reino Unido