Effects of CSF1R/p-ERK1/2 signaling pathway on RF/6A cells under high glucose conditions.
Eur J Ophthalmol
; 34(4): 1165-1173, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38099815
ABSTRACT
OBJECTIVE:
This study analyzed how high glucose affects CSF1R and p-ERK1/2 expression in RF/6A cells.METHODS:
The cells were cultured as high glucose (HG) and normal control (C) groups, and CSF1R shRNA was introduced. Real time PCR was used to detect the expression of CSF1R and p-ERK1/2 mRNA. Western blot was used to detect the expression of CSF1R and p-ERK1/2 proteins. Cell Counting Kit 8 (CCK-8) method was used to detect cell proliferation, while flow cytometry was used to detect apoptosis in HREC.RESULTS:
Real-time PCR showed significantly raised CSF1R mRNA expression in HG. CSF1R inhibition lowered HG + LV shCSF1R CSF1R mRNA levels. Western blotting revealed higher CSF1R and p-ERK1/2 protein expression in HG than in C. Their expression level dropped after CSF1R inhibition. The number of tube-forming cells was higher in HG than in C, which reduced after CSF1R suppression. Inhibiting CSF1R also decreased cell proliferation and raised apoptosis.CONCLUSION:
Overall, under high glucose, CSF1R and p-ERK1/2 were highly expressed, leading to reduced cellular activity, and CSF1R inhibition helped alleviate this effect.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Western Blotting
/
Apoptosis
/
Receptores de Factor Estimulante de Colonias de Granulocitos y Macrófagos
/
Sistema de Señalización de MAP Quinasas
/
Proliferación Celular
/
Glucosa
Límite:
Animals
Idioma:
En
Revista:
Eur J Ophthalmol
Asunto de la revista:
OFTALMOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Estados Unidos