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High risk HPV-positive women cervicovaginal microbial profiles in a Greek cohort: a retrospective analysis of the GRECOSELF study.
Sofou, Electra; Gkoliou, Glykeria; Pechlivanis, Nikolaos; Pasentsis, Konstantinos; Chatzistamatiou, Kimon; Psomopoulos, Fotis; Agorastos, Theodoros; Stamatopoulos, Kostas.
Afiliación
  • Sofou E; Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, Greece.
  • Gkoliou G; Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, Greece.
  • Pechlivanis N; Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupoli, Greece.
  • Pasentsis K; Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, Greece.
  • Chatzistamatiou K; Department of Genetics, Development and Molecular Biology, Faculty of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece.
  • Psomopoulos F; Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, Greece.
  • Agorastos T; 1st Department of Obstetrics and Gynecology, Aristotle University of Thessaloniki, Papageorgiou General Hospital, Thessaloniki, Greece.
  • Stamatopoulos K; Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki, Greece.
Front Microbiol ; 14: 1292230, 2023.
Article en En | MEDLINE | ID: mdl-38098662
ABSTRACT
Increasing evidence supports a role for the vaginal microbiome (VM) in the severity of HPV infection and its potential link to cervical intraepithelial neoplasia. However, a lot remains unclear regarding the precise role of certain bacteria in the context of HPV positivity and persistence of infection. Here, using next generation sequencing (NGS), we comprehensively profiled the VM in a series of 877 women who tested positive for at least one high risk HPV (hrHPV) type with the COBAS® 4,800 assay, after self-collection of a cervico-vaginal sample. Starting from gDNA, we PCR amplified the V3-V4 region of the bacterial 16S rRNA gene and applied a paired-end NGS protocol (Illumina). We report significant differences in the abundance of certain bacteria compared among different HPV-types, more particularly concerning species assigned to Lacticaseibacillus, Megasphaera and Sneathia genera. Especially for Lacticaseibacillus, we observed significant depletion in the case of HPV16, HPV18 versus hrHPVother. Overall, our results suggest that the presence or absence of specific cervicovaginal microbial genera may be linked to the observed severity in hrHPV infection, particularly in the case of HPV16, 18 types.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2023 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2023 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Suiza