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A Reproducible Cartilage Impact Model to Generate Post-Traumatic Osteoarthritis in the Rabbit.
Dilley, Julian; Noori-Dokht, Hessam; Seetharam, Abhijit; Bello, Margaret; Nanavaty, Aaron; Natoli, Roman M; McKinley, Todd; Bault, Zachary; Wagner, Diane; Sankar, Uma.
Afiliación
  • Dilley J; Department of Anatomy, Cell Biology and Physiology, Indiana University School of Medicine; Department of Orthopaedic Surgery, Indiana University School of Medicine; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine.
  • Noori-Dokht H; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine; School of Mechanical Engineering, Purdue University; Department of Mechanical and Energy Engineering, Indiana University-Purdue University Indianapolis.
  • Seetharam A; Department of Anatomy, Cell Biology and Physiology, Indiana University School of Medicine.
  • Bello M; Department of Anatomy, Cell Biology and Physiology, Indiana University School of Medicine; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine.
  • Nanavaty A; Department of Anatomy, Cell Biology and Physiology, Indiana University School of Medicine; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine.
  • Natoli RM; Department of Orthopaedic Surgery, Indiana University School of Medicine; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine.
  • McKinley T; Department of Anatomy, Cell Biology and Physiology, Indiana University School of Medicine; Department of Orthopaedic Surgery, Indiana University School of Medicine; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine.
  • Bault Z; Large Animal Resource Center, Indiana University School of Medicine.
  • Wagner D; Department of Orthopaedic Surgery, Indiana University School of Medicine; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine; Department of Mechanical and Energy Engineering, Indiana University-Purdue University Indianapolis; wagnerdi@iupui.edu.
  • Sankar U; Department of Anatomy, Cell Biology and Physiology, Indiana University School of Medicine; Indiana Center for Musculoskeletal Health, Indiana University School of Medicine; usankar@iupui.edu.
J Vis Exp ; (201)2023 Nov 21.
Article en En | MEDLINE | ID: mdl-38078617
Post-traumatic osteoarthritis (PTOA) is responsible for 12% of all osteoarthritis cases in the United States. PTOA can be initiated by a single traumatic event, such as a high-impact load acting on articular cartilage, or by joint instability, as occurs with anterior cruciate ligament rupture. There are no effective therapeutics to prevent PTOA currently. Developing a reliable animal model of PTOA is necessary to better understand the mechanisms by which cartilage damage proceeds and to investigate novel treatment strategies to alleviate or prevent the progression of PTOA. This protocol describes an open, drop tower-based rabbit femoral condyle impact model to induce cartilage damage. This model delivered peak loads of 579.1 ± 71.1 N, and peak stresses of 81.9 ± 10.1 MPa with a time-to-peak load of 2.4 ± 0.5 ms. Articular cartilage from impacted medial femoral condyles (MFCs) had higher rates of apoptotic cells (p = 0.0058) and possessed higher Osteoarthritis Research Society International (OARSI) scores of 3.38 ± 1.43 compared to the non-impacted contralateral MFCs (0.56 ± 0.42), and other cartilage surfaces of the impacted knee (p < 0.0001). No differences in OARSI scores were detected among the non-impacted articular surfaces (p > 0.05).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoartritis / Cartílago Articular / Lesiones del Ligamento Cruzado Anterior Límite: Animals Idioma: En Revista: J Vis Exp Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoartritis / Cartílago Articular / Lesiones del Ligamento Cruzado Anterior Límite: Animals Idioma: En Revista: J Vis Exp Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos