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A series of caged fluorophores for calibrating light intensity.
Mandal, Mrinal; Tehrani, Hessam Sepasi; Mai, Qianhua; Simon, Emma; Plamont, Marie-Aude; Rampon, Christine; Vriz, Sophie; Aujard, Isabelle; Le Saux, Thomas; Jullien, Ludovic.
Afiliación
  • Mandal M; PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS 24, rue Lhomond 75005 Paris France Isabelle.Aujard@ens.psl.eu Thomas.Lesaux@ens.psl.eu Ludovic.Jullien@ens.psl.eu.
  • Tehrani HS; PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS 24, rue Lhomond 75005 Paris France Isabelle.Aujard@ens.psl.eu Thomas.Lesaux@ens.psl.eu Ludovic.Jullien@ens.psl.eu.
  • Mai Q; PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS 24, rue Lhomond 75005 Paris France Isabelle.Aujard@ens.psl.eu Thomas.Lesaux@ens.psl.eu Ludovic.Jullien@ens.psl.eu.
  • Simon E; PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS 24, rue Lhomond 75005 Paris France Isabelle.Aujard@ens.psl.eu Thomas.Lesaux@ens.psl.eu Ludovic.Jullien@ens.psl.eu.
  • Plamont MA; PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS 24, rue Lhomond 75005 Paris France Isabelle.Aujard@ens.psl.eu Thomas.Lesaux@ens.psl.eu Ludovic.Jullien@ens.psl.eu.
  • Rampon C; Laboratoire des biomolécules (LBM), Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS 24, rue Lhomond 75005 Paris France.
  • Vriz S; Laboratoire des biomolécules (LBM), Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS 24, rue Lhomond 75005 Paris France.
  • Aujard I; PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS 24, rue Lhomond 75005 Paris France Isabelle.Aujard@ens.psl.eu Thomas.Lesaux@ens.psl.eu Ludovic.Jullien@ens.psl.eu.
  • Le Saux T; PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS 24, rue Lhomond 75005 Paris France Isabelle.Aujard@ens.psl.eu Thomas.Lesaux@ens.psl.eu Ludovic.Jullien@ens.psl.eu.
  • Jullien L; PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS 24, rue Lhomond 75005 Paris France Isabelle.Aujard@ens.psl.eu Thomas.Lesaux@ens.psl.eu Ludovic.Jullien@ens.psl.eu.
Chem Sci ; 14(47): 13799-13811, 2023 Dec 06.
Article en En | MEDLINE | ID: mdl-38075640
Absolute measurement of light intensity is sought for in multiple areas of chemistry, biology, physics, and engineering. It can be achieved by using an actinometer from analyzing the time-course of its reaction extent on applying constant light. However, most reported actinometers exploit the absorbance observable for reporting the reaction extent, which is not very sensitive nor relevant in imaging systems. In this work, we report a series of hydrophobic and hydrophilic caged fluorophores that overcome the preceding limitations. Based on the robust pyranine backbone, they can easily be synthesized on a large scale in one to a few steps. Their brightness increases over illumination and their uncaging cross-sections have been thoroughly characterized upon one- and two-photon excitation. As a demonstration of their use, we calibrated light intensity in various chemical and biological samples, which have been observed with epifluorescence and confocal imaging systems.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Sci Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Sci Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido