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Bioconversion of citrus waste into mucic acid by xylose-fermenting Saccharomyces cerevisiae.
Jeong, Deokyeol; Park, Sujeong; Evelina, Grace; Kim, Suhyeung; Park, Heeyoung; Lee, Je Min; Kim, Sun-Ki; Kim, In Jung; Oh, Eun Joong; Kim, Soo Rin.
Afiliación
  • Jeong D; Department of Food Science, Purdue University, West Lafayette, IN 47907, United State.
  • Park S; School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
  • Evelina G; School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
  • Kim S; School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
  • Park H; School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
  • Lee JM; Department of Horticultural Science, Kyungpook National University, Daegu 41566, Republic of Korea.
  • Kim SK; Department of Food Science and Technology, Chung-Ang University, Anseong, Gyeonggi 17546, Republic of Korea.
  • Kim IJ; Department of Food Science & Technology, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea.
  • Oh EJ; Department of Food Science, Purdue University, West Lafayette, IN 47907, United State. Electronic address: ejoh@purdue.edu.
  • Kim SR; School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea; Research Institute of Tailored Food Technology, Kyungpook National University, Daegu 41566, Republic of Korea. Electronic address: soorinkim@knu.ac.kr.
Bioresour Technol ; 393: 130158, 2024 Feb.
Article en En | MEDLINE | ID: mdl-38070579
Mucic acid holds promise as a platform chemical for bio-based nylon synthesis; however, its biological production encounters challenges including low yield and productivity. In this study, an efficient and high-yield method for mucic acid production was developed by employing genetically engineered Saccharomyces cerevisiae expressing the NAD+-dependent uronate dehydrogenase (udh) gene. To overcome the NAD+ dependency for the conversion of pectin to mucic acid, xylose was utilized as a co-substrate. Through optimization of the udh expression system, the engineered strain achieved a notable output, producing 20 g/L mucic acid with a highest reported productivity of 0.83 g/L-h and a theoretical yield of 0.18 g/g when processing pectin-containing citrus peel waste. These results suggest promising industrial applications for the biological production of mucic acid. Additionally, there is potential to establish a viable bioprocess by harnessing pectin-rich fruit waste alongside xylose-rich cellulosic biomass as raw materials.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Azúcares Ácidos / Citrus Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Azúcares Ácidos / Citrus Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido