Phenotypic Analysis of Early Neurogenesis in a Mouse Chimeric Embryo and Stem Cell-Based Neuruloid Model.

McMahon, Riley; Masamsetti, V Pragathi; Tam, Patrick P L

McMahon, Riley; Masamsetti, V Pragathi; Tam, Patrick P L.

Afiliación

McMahon R; Embryology Research Unit, Children's Medical Research Institute, University of Sydney, Westmead, NSW, Australia.
Masamsetti VP; School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia.
Tam PPL; Embryology Research Unit, Children's Medical Research Institute, University of Sydney, Westmead, NSW, Australia.

Methods Mol Biol ; 2746: 165-177, 2024.

Article en En | MEDLINE | ID: mdl-38070089

ABSTRACT

ABSTRACT

Analyzing the impact of genetic mutations on early neurogenesis of mammalian embryos in conventional mouse mutant models is laborious and time-consuming. To overcome these constraints and to fast-track the phenotypic analysis, we developed a protocol that harnesses the amenability of engineering genetic modifications in embryonic stem cells from which mid-gestation mouse chimeras and in vitro neuruloids are generated. These stem cell-based chimera and neuruloid experimental models allow phenotyping at early developmental time points of neurogenesis.

Asunto(s)

Embrión de Mamíferos; Células Madre Embrionarias; Ratones; Animales; Neurogénesis/genética; Mamíferos

Palabras clave

Confocal microscopy; Embryos; Immunostaining; Neurogenesis; Stem cells

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Embrión de Mamíferos / Células Madre Embrionarias Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google