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PDGFRß Activation Induced the Bovine Embryonic Genome Activation via Enhanced NFYA Nuclear Localization.
Perera, Chalani Dilshani; Idrees, Muhammad; Khan, Abdul Majid; Haider, Zaheer; Ullah, Safeer; Kang, Ji-Su; Lee, Seo-Hyun; Kang, Seon-Min; Kong, Il-Keun.
Afiliación
  • Perera CD; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea.
  • Idrees M; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea.
  • Khan AM; Institute of Agriculture and Life Science (IALS), Gyeongsang National University, Jinju 52828, Republic of Korea.
  • Haider Z; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea.
  • Ullah S; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea.
  • Kang JS; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea.
  • Lee SH; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea.
  • Kang SM; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea.
  • Kong IK; Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea.
Int J Mol Sci ; 24(23)2023 Dec 01.
Article en En | MEDLINE | ID: mdl-38069370
Embryonic genome activation (EGA) is a critical step during embryonic development. Several transcription factors have been identified that play major roles in initiating EGA; however, this gradual and complex mechanism still needs to be explored. In this study, we investigated the role of nuclear transcription factor Y subunit A (NFYA) in bovine EGA and bovine embryonic development and its relationship with the platelet-derived growth factor receptor-ß (PDGFRß) by using a potent selective activator (PDGF-BB) and inhibitor (CP-673451) of PDGF receptors. Activation and inhibition of PDGFRß using PDGF-BB and CP-673451 revealed that NFYA expression is significantly (p < 0.05) affected by the PDGFRß. In addition, PDGFRß mRNA expression was significantly increased (p < 0.05) in the activator group and significantly decreased (p < 0.05) in the inhibitor group when compared with PDGFRα. Downregulation of NFYA following PDGFRß inhibition was associated with the expression of critical EGA-related genes, bovine embryo development rate, and implantation potential. Moreover, ROS and mitochondrial apoptosis levels and expression of pluripotency-related markers necessary for inner cell mass development were also significantly (p < 0.05) affected by the downregulation of NFYA while interrupting trophoblast cell (CDX2) differentiation. In conclusion, the PDGFRß-NFYA axis is critical for bovine embryonic genome activation and embryonic development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Receptor beta de Factor de Crecimiento Derivado de Plaquetas Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Receptor beta de Factor de Crecimiento Derivado de Plaquetas Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article Pais de publicación: Suiza