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HPLC-DAD-MS Characterization, Antioxidant Activity, α-amylase Inhibition, Molecular Docking, and ADMET of Flavonoids from Fenugreek Seeds.
Khenifi, Mohammed Lamine; Serseg, Talia; Migas, Piotr; Krauze-Baranowska, Miroslawa; Özdemir, Sadin; Bensouici, Chawki; Alghonaim, Mohammed I; Al-Khafaji, Khattab; Alsalamah, Sulaiman A; Boudjeniba, Messaoud; Yousfi, Mohamed; Boufahja, Fehmi; Bendif, Hamdi; Mahdid, Mohamed.
Afiliación
  • Khenifi ML; Laboratoire d'Ethnobotanique et Substances Naturelles, Departement of Biology, Ecole Normale Supérieure de Kouba, B.P 92, Kouba 16308, Algeria.
  • Serseg T; Laboratoire des Sciences Appliquées et Didactiques, Ecole Normale Supérieure de Laghouat, B.P 4033 la gare routière, Laghouat 03000, Algeria.
  • Migas P; Laboratoire des Sciences Appliquées et Didactiques, Ecole Normale Supérieure de Laghouat, B.P 4033 la gare routière, Laghouat 03000, Algeria.
  • Krauze-Baranowska M; Fundamental Sciences Laboratory, Amar Telidji University, Laghouat 03000, Algeria.
  • Özdemir S; Pharmacognosy with Medicinal Plants Garden, Medical University of Gdansk, 80-416 Gdansk, Poland.
  • Bensouici C; Pharmacognosy with Medicinal Plants Garden, Medical University of Gdansk, 80-416 Gdansk, Poland.
  • Alghonaim MI; Food Processing Programme, Technical Science Vocational School Mersin University, Mersin TR-33343, Turkey.
  • Al-Khafaji K; Biotechnology Research Center (CRBT), Ali Mendjli New Town UV 03, B.P E73, Constantine 25016, Algeria.
  • Alsalamah SA; Biology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia.
  • Boudjeniba M; Department of Environmental Science, College of Energy and Environmental Science, Al-Karkh University of Science, Baghdad 10081, Iraq.
  • Yousfi M; Biology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia.
  • Boufahja F; Laboratoire d'Ethnobotanique et Substances Naturelles, Departement of Biology, Ecole Normale Supérieure de Kouba, B.P 92, Kouba 16308, Algeria.
  • Bendif H; Fundamental Sciences Laboratory, Amar Telidji University, Laghouat 03000, Algeria.
  • Mahdid M; Biology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia.
Molecules ; 28(23)2023 Nov 27.
Article en En | MEDLINE | ID: mdl-38067527
Fenugreek (Trigonella foenum-graecum) has a great beneficial health effect; it has been used in traditional medicine by many cultures. Likewise, the α-amylase inhibitors are potential compounds in the development of drugs for the treatment of diabetes. The beneficial health effects of fenugreek lead us to explore the chemical composition of the seeds and their antioxidant and α-amylase inhibition activities. The flavonoid extraction from fenugreek seeds was achieved with methanol through a Soxhlet apparatus. Then, the flavonoid glycosides were characterized using HPLC-DAD-ESI-MS analysis. The antioxidant capacity of fenugreek seed was measured using DPPH, FRAP, ABTS, and CUPRAC assays. Finally, the α-amylase inhibition activity was carried out using in vitro and in silico methods. The methanolic extract was found to contain high amounts of total phenolics (154.68 ± 1.50 µg GAE/mg E), flavonoids (37.69 ± 0.73 µg QE/mg E). The highest radical-scavenging ability was recorded for the methanolic extract against DPPH (IC50 = 556.6 ± 9.87 µg/mL), ABTS (IC50 = 593.62 ± 9.35 µg/mL). The ME had the best reducing power according to the CUPRAC (A 0.5 = 451.90 ± 9.07 µg/mL). The results indicate that the methanolic extracts of fenugreek seed best α-amylase inhibition activities IC50 = 653.52 ± 3.24 µg/mL. Twenty-seven flavonoids were detected, and all studied flavonoids selected have good affinity and stabilize very well in the pocket of α-amylase. The interactions between the studied flavonoids with α-amylase were investigated. The flavonoids from fenugreek seed present a good inhibitory effect against α-amylase, which is beneficial for the prevention of diabetes and its complications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trigonella / Diabetes Mellitus Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Argelia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trigonella / Diabetes Mellitus Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Argelia Pais de publicación: Suiza