Kinetic FRET Assay to Measure Binding-Induced Conformational Changes of Nucleic Acids.

Higuera-Rodriguez, R Anahi; De Pascali, Mareike C; Aziz, Masood; Sattler, Michael; Rant, Ulrich; Kaiser, Wolfgang

Higuera-Rodriguez, R Anahi; De Pascali, Mareike C; Aziz, Masood; Sattler, Michael; Rant, Ulrich; Kaiser, Wolfgang.

Afiliación

Higuera-Rodriguez RA; TUM School of Natural Sciences, Department of Bioscience, Technical University of Munich, Garching 85748, Germany.
De Pascali MC; Dynamic Biosensors GmbH, Perchtinger Str. 8/10, Munich 81379, Germany.
Aziz M; TUM School of Natural Sciences, Department of Bioscience, Technical University of Munich, Garching 85748, Germany.
Sattler M; Dynamic Biosensors GmbH, Perchtinger Str. 8/10, Munich 81379, Germany.
Rant U; TUM School of Natural Sciences, Department of Bioscience, Technical University of Munich, Garching 85748, Germany.
Kaiser W; Helmholtz Munich, Molecular Targets and Therapeutics Center, Institute of Structural Biology, Neuherberg 85764, Germany.

ACS Sens ; 8(12): 4597-4606, 2023 Dec 22.

Article en En | MEDLINE | ID: mdl-38060303

Asunto(s)

Transferencia Resonante de Energía de Fluorescencia; Ácidos Nucleicos; Humanos; Transferencia Resonante de Energía de Fluorescencia/métodos; ARN/química; Proteínas; ADN/química

Palabras clave

Förster resonance energy transfer; RNA binding protein; aptamer; binding kinetics; biosensors; nucleic acid conformational changes; switchSENSE technology

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Nucleicos / Transferencia Resonante de Energía de Fluorescencia Límite: Humans Idioma: En Revista: ACS Sens Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

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