Your browser doesn't support javascript.
loading
Intramuscular but not nebulized administration of a mRNA vaccine against Rhodococcus equi stimulated humoral immune responses in neonatal foals.
Legere, Rebecca M; Poveda, Cristina; Ott, Jeannine A; Bray, Jocelyne M; Villafone, Emma G; Silveira, Bibiana Petri da; Kahn, Susanne K; Martin, Cameron L; Mancino, Chiara; Taraballi, Francesca; Criscitiello, Michael F; Berghman, Luc; Bordin, Angela I; Pollet, Jeroen; Cohen, Noah D.
Afiliación
  • Legere RM; Equine Infectious Disease Laboratory, Department of Large Animal Clinical Sciences, School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX.
  • Poveda C; Department of Pediatrics, Division of Tropical Medicine, Baylor College of Medicine, Houston, TX.
  • Ott JA; Texas Children's Hospital Center for Vaccine Development, Houston, TX.
  • Bray JM; Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX.
  • Villafone EG; Equine Infectious Disease Laboratory, Department of Large Animal Clinical Sciences, School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX.
  • Silveira BPD; Equine Infectious Disease Laboratory, Department of Large Animal Clinical Sciences, School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX.
  • Kahn SK; Equine Infectious Disease Laboratory, Department of Large Animal Clinical Sciences, School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX.
  • Martin CL; Equine Infectious Disease Laboratory, Department of Large Animal Clinical Sciences, School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX.
  • Mancino C; Department of Poultry Science, College of Agriculture & Life Sciences, Texas A&M University, College Station, TX.
  • Taraballi F; Center for Musculoskeletal Regeneration, Houston Methodist Research Institute, Houston, TX.
  • Criscitiello MF; Orthopedics and Sports Medicine, Houston Methodist Hospital, Houston, TX.
  • Berghman L; Center for Musculoskeletal Regeneration, Houston Methodist Research Institute, Houston, TX.
  • Bordin AI; Orthopedics and Sports Medicine, Houston Methodist Hospital, Houston, TX.
  • Pollet J; Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX.
  • Cohen ND; Comparative Immunogenetics Laboratory, Department of Veterinary Pathobiology, School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX.
Am J Vet Res ; 85(2)2024 Feb 01.
Article en En | MEDLINE | ID: mdl-38056076
OBJECTIVE: Design and evaluate immune responses of neonatal foals to a mRNA vaccine expressing the virulence-associated protein A (VapA) of Rhodococcus equi. ANIMALS: Cultured primary equine respiratory tract cells; Serum, bronchoalveolar lavage fluid (BALF), and peripheral blood mononuclear cells (PBMCs) from 30 healthy Quarter Horse foals. METHODS: VapA expression was evaluated by western immunoblot in cultured equine bronchial cells transfected with 4 mRNA constructs encoding VapA. The mRNA construct with greatest expression was used to immunize foals at ages 2 and 21 days in 5 groups: (1) 300 µg nebulized mRNA (n = 6); (2) 600 µg nebulized mRNA (n = 4); (3) 300 µg mRNA administered intramuscularly (IM) (n = 5); (4) 300 µg VapA IM (positive controls; n = 6); or (5) nebulized water (negative controls; n = 6). Serum, BALF, and PBMCs were collected at ages 3, 22, and 35 days and tested for relative anti-VapA IgG1, IgG4/7, and IgA activities using ELISA and cell-mediated immunity by ELISpot. RESULTS: As formulated, nebulized mRNA was not immunogenic. However, a significant increase in anti-VapA IgG4/7 activity (P < .05) was noted exclusively in foals immunized IM with VapA mRNA by age 35 days. The proportion of foals with anti-VapA IgG1 activity > 30% of positive control differed significantly (P = .0441) between negative controls (50%; 3/6), IM mRNA foals (100%; 5/5), and IM VapA (100%; 6/6) groups. Natural exposure to virulent R equi was immunogenic in some negative control foals. CLINICAL RELEVANCE: Further evaluation of the immunogenicity and efficacy of IM mRNA encoding VapA in foals is warranted.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Actinomycetales / Rhodococcus equi / Enfermedades de los Caballos Límite: Animals Idioma: En Revista: Am J Vet Res Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Actinomycetales / Rhodococcus equi / Enfermedades de los Caballos Límite: Animals Idioma: En Revista: Am J Vet Res Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos