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Diversity, molecular phylogenetics, and antibiotic biosynthetic potential of endophytic Actinobacteria isolated from medicinal plants in Nigeria.
Ezeobiora, Chijioke E; Igbokwe, Nwamaka H; Okpalanwa, Chiamaka F; Stephen, Chabula M; Mendie, Udoma E; Amin, Dina H.
Afiliación
  • Ezeobiora CE; Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria. chijiokeemmanuel177@yahoo.com.
  • Igbokwe NH; Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria.
  • Okpalanwa CF; Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Nigeria, Nsukka, Nigeria.
  • Stephen CM; Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Maiduguri, Maiduguri, Borno, Nigeria.
  • Mendie UE; Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria.
  • Amin DH; Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt.
Braz J Microbiol ; 55(1): 179-190, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38030865
Actinobacteria that are found in nature have enormous promise for the growth of the pharmaceutical sector. There is a scarce report on the antimicrobial activities of endophytic Actinobacteria from Nigeria. As a result, this study evaluated the Actinobacteria isolated from Nigerian medicinal plants in terms of their biodiversity, phylogenetics, and ability to produce antimicrobial compounds. Following accepted practices, Actinobacteria were isolated from the surface-sterilized plant parts. They were identified using 16S rRNA sequencing, microscopic, and morphological methods. The cell-free broth of Actinobacteria isolates was subjected to antimicrobial assay by agar well diffusion. Molecular evolutionary and genetic analysis (MEGA) version X was used for phylogenetic analysis, and the interactive tree of life (iTOL) version 6.0 was used to view the neighbour-joining method-drawn tree. A total of 13 Actinobacteria were recovered, belonging to three genera including 10 strains of Streptomyces, 2 strains of Saccharomonospora, and only 1 strain of Saccharopolyspora. They showed inhibitory activity against several bacterial pathogens. The phylogenetic tree generated from the sequences showed that our isolates are divergent and distinct from other closely related strains on the database. Further, optimization of the antibiotic production by selected Saccharomonospora sp. PNSac2 was conducted. It showed that the optimal conditions were the ISP2 medium (1-2% w/v salt) adjusted to pH of 8 at 30-32℃ for 12-14 days. In conclusion, endophytic Actinobacteria dwelling in Nigerian soils could be a promising source of new antibiotics. Future research is warranted because more genomic analysis and characterization of their metabolites could lead to the development of new antibacterial medicines.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plantas Medicinales / Streptomyces / Actinobacteria / Antiinfecciosos País/Región como asunto: Africa Idioma: En Revista: Braz J Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Nigeria Pais de publicación: Brasil
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plantas Medicinales / Streptomyces / Actinobacteria / Antiinfecciosos País/Región como asunto: Africa Idioma: En Revista: Braz J Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Nigeria Pais de publicación: Brasil