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Resolving an inconsistency in the estimation of the energy for excitation of cardiac muscle contraction.
Han, June-Chiew; Pham, Toan; Taberner, Andrew J; Loiselle, Denis S; Tran, Kenneth.
Afiliación
  • Han JC; Auckland Bioengineering Institute, The University of Auckland, Auckland, New Zealand.
  • Pham T; Auckland Bioengineering Institute, The University of Auckland, Auckland, New Zealand.
  • Taberner AJ; Auckland Bioengineering Institute, The University of Auckland, Auckland, New Zealand.
  • Loiselle DS; Department of Engineering Science and Biomedical Engineering, The University of Auckland, Auckland, New Zealand.
  • Tran K; Auckland Bioengineering Institute, The University of Auckland, Auckland, New Zealand.
Front Physiol ; 14: 1269900, 2023.
Article en En | MEDLINE | ID: mdl-38028799
In the excitation of muscle contraction, calcium ions interact with transmembrane transporters. This process is accompanied by energy consumption and heat liberation. To quantify this activation energy or heat in the heart or cardiac muscle, two non-pharmacological approaches can be used. In one approach using the "pressure-volume area" concept, the same estimate of activation energy is obtained regardless of the mode of contraction (either isovolumic/isometric or ejecting/shortening). In the other approach, an accurate estimate of activation energy is obtained only when the muscle contracts isometrically. If the contraction involves muscle shortening, then an additional component of heat associated with shortening is liberated, over and above that of activation. The present study thus examines the reconcilability of the two approaches by performing experiments on isolated muscles measuring contractile force and heat output. A framework was devised from the experimental data to allow us to replicate several mechanoenergetics results gleaned from the literature. From these replications, we conclude that the choice of initial muscle length (or ventricular volume) underlies the divergence of the two approaches in the estimation of activation energy when the mode of contraction involves shortening (ejection). At low initial muscle lengths, the heat of shortening is relatively small, which can lead to the misconception that activation energy is contraction mode independent. In fact, because cardiac muscle liberates heat of shortening when allowed to shorten, estimation of activation heat must be performed only under isometric (isovolumic) contractions. We thus recommend caution when estimating activation energy using the "pressure-volume area" concept.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Physiol Año: 2023 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Physiol Año: 2023 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Suiza