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A rat liver cell atlas reveals intrahepatic myeloid heterogeneity.
Pouyabahar, Delaram; Chung, Sai W; Pezzutti, Olivia I; Perciani, Catia T; Wang, Xinle; Ma, Xue-Zhong; Jiang, Chao; Camat, Damra; Chung, Trevor; Sekhon, Manmeet; Manuel, Justin; Chen, Xu-Chun; McGilvray, Ian D; MacParland, Sonya A; Bader, Gary D.
Afiliación
  • Pouyabahar D; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
  • Chung SW; The Donnelly Centre, University of Toronto, Toronto, ON, Canada.
  • Pezzutti OI; Ajmera Transplant Centre, Toronto General Hospital Research Institute, Toronto, ON, Canada.
  • Perciani CT; Department of Immunology, University of Toronto, Toronto, ON, Canada.
  • Wang X; Ajmera Transplant Centre, Toronto General Hospital Research Institute, Toronto, ON, Canada.
  • Ma XZ; Ajmera Transplant Centre, Toronto General Hospital Research Institute, Toronto, ON, Canada.
  • Jiang C; Ajmera Transplant Centre, Toronto General Hospital Research Institute, Toronto, ON, Canada.
  • Camat D; Ajmera Transplant Centre, Toronto General Hospital Research Institute, Toronto, ON, Canada.
  • Chung T; Ajmera Transplant Centre, Toronto General Hospital Research Institute, Toronto, ON, Canada.
  • Sekhon M; Ajmera Transplant Centre, Toronto General Hospital Research Institute, Toronto, ON, Canada.
  • Manuel J; Department of Immunology, University of Toronto, Toronto, ON, Canada.
  • Chen XC; Ajmera Transplant Centre, Toronto General Hospital Research Institute, Toronto, ON, Canada.
  • McGilvray ID; Ajmera Transplant Centre, Toronto General Hospital Research Institute, Toronto, ON, Canada.
  • MacParland SA; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
  • Bader GD; Ajmera Transplant Centre, Toronto General Hospital Research Institute, Toronto, ON, Canada.
iScience ; 26(11): 108213, 2023 Nov 17.
Article en En | MEDLINE | ID: mdl-38026201
The large size and vascular accessibility of the laboratory rat (Rattus norvegicus) make it an ideal hepatic animal model for diseases that require surgical manipulation. Often, the disease susceptibility and outcomes of inflammatory pathologies vary significantly between strains. This study uses single-cell transcriptomics to better understand the complex cellular network of the rat liver, as well as to unravel the cellular and molecular sources of inter-strain hepatic variation. We generated single-cell and single-nucleus transcriptomic maps of the livers of healthy Dark Agouti and Lewis rat strains and developed a factor analysis-based bioinformatics analysis pipeline to study data covariates, such as strain and batch. Using this approach, we discovered transcriptomic variation within the hepatocyte and myeloid populations that underlie distinct cell states between rat strains. This finding will help provide a reference for future investigations on strain-dependent outcomes of surgical experiment models.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: IScience Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: IScience Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos