Introduction: Performance analysis through game-related statistics in wheelchair basketball (WB) has focused mainly on the study of the individual efficiency of players according to their functional classification. However, there is little evidence focusing on lineup performances (five players on court) and their composition. Thus, the objective of present study was to analyze the efficiency of the women's WB lineups used during the Tokyo 2020 Paralympic Games (PG) and to determine the variables that best discriminated the lineup performances according to the final point differential. Methods: The sample comprised 507 lineups used in the 31 games by the 10 national teams during the competition. Fifty-one different lineup types (LTs) were categorized. A discriminant analysis was carried out to compare the lineups with a positive and negative point difference according to the game type (balanced and unbalanced games). Results: It was found that LTs 16 (1-1.5-2.5-4-4.5), 47 (1-2-2.5-4-4.5) and 14 (1-1.5-2.5-4.5-4.5) had the best means of efficiency in field goals (LT 16 = 52%; LT 47 = 44% and LT = 40%), while LT 50 (1-2-3-4-4) obtained the highest mean difference in points (3.67 ± 10.67). The variables that best discriminated winner teams in balanced games were field goal efficiency (SC = 0.55), assists (SC = 0.50) and turnovers (SC = -0.41). Discussion: Field goal efficiency, assists, turnovers and steals are the game-related statistics most associated with the success of a lineup used in balanced games in WB in PG competition; this could be taken into account by coaches when deciding how to compose a given lineup in a moment of the game, to adequately select players from different functional classifications for the final squad and to choose training content related to the indicated game-related statistics, as they explain success at this competition level.