Selection and characterization of ssDNA aptamer targeting Macrobrachium rosenbergii nodavirus capsid protein: A potential capture agent in gold-nanoparticle-based aptasensor for viral protein detection.

Ghadin, Norazli; Yusof, Nur Afiqah Md; Syarul Nataqain, Baharum; Raston, Nurul Hanun Ahmad; Low, Chen Fei

Ghadin, Norazli; Yusof, Nur Afiqah Md; Syarul Nataqain, Baharum; Raston, Nurul Hanun Ahmad; Low, Chen Fei.

Afiliación

Ghadin N; Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
Yusof NAM; Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
Syarul Nataqain B; Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
Raston NHA; Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
Low CF; Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.

J Fish Dis ; 47(2): e13892, 2024 Feb.

Article en En | MEDLINE | ID: mdl-38014615

RESUMEN

The giant freshwater prawn holds a significant position as a valuable crustacean species cultivated in the aquaculture industry, particularly well-known and demanded among the Southeast Asian countries. Aquaculture production of this species has been impacted by Macrobrachium rosenbergii nodavirus (MrNV) infection, which particularly affects the larvae and post-larvae stages of the prawn. The infection has been recorded to cause mortality rates of up to 100% among the affected prawns. A simple, fast, and easy to deploy on-site detection or diagnostic method is crucial for early detection of MrNV to control the disease outbreak. In the present study, novel single-stranded DNA aptamers targeting the MrNV capsid protein were identified using the systematic evolution of ligands by exponential enrichment (SELEX) approach. The aptamer was then conjugated with the citrate-capped gold nanoparticles (AuNPs), and the sensitivity of this AuNP-based aptasensor for the detection of MrNV capsid protein was evaluated. Findings revealed that the aptamer candidate, APT-MrNV-CP-1 was enriched throughout the SELEX cycle 4, 9, and 12 with the sequence percentage of 1.76%, 9.09%, and 12.42%, respectively. The conjugation of APT-MrNV-CP-1 with citrate-capped AuNPs exhibited the highest sensitivity in detecting the MrNV capsid protein, where the presence of 62.5 nM of the viral capsid protein led to a significant agglomeration of the AuNPs. This study demonstrated the practicality of an AuNP-based aptasensor for disease diagnosis, particularly for detecting MrNV infection in giant freshwater prawns.

Asunto(s)

Enfermedades de los Peces; Nanopartículas del Metal; Nodaviridae; Palaemonidae; Animales; Proteínas de la Cápside/genética; Proteínas de la Cápside/metabolismo; Palaemonidae/genética; Proteínas Virales/genética; Oro; ADN de Cadena Simple; Enfermedades de los Peces/diagnóstico; Nodaviridae/genética; Citratos/metabolismo

Palabras clave

Macrobrachium rosenbergii nodavirus; gold nanoparticle; ssDNA aptamer; viral capsid protein

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nodaviridae / Palaemonidae / Nanopartículas del Metal / Enfermedades de los Peces Límite: Animals Idioma: En Revista: J Fish Dis Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Reino Unido

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