Your browser doesn't support javascript.
loading
Drug repurposing screens identify Tubercidin as a potent antiviral agent against porcine nidovirus infections.
Wang, Tianliang; Zheng, Guanmin; Chen, Zilu; Wang, Yue; Zhao, Chenxu; Li, Yaqin; Yuan, Yixin; Duan, Hong; Zhu, Hongsen; Yang, Xia; Li, Wentao; Du, Wenjuan; Li, Yongtao; Li, Dongliang.
Afiliación
  • Wang T; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
  • Zheng G; School of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China.
  • Chen Z; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
  • Wang Y; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
  • Zhao C; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
  • Li Y; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
  • Yuan Y; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
  • Duan H; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Agricultural University, Zhengzhou, Henan 450046, China.
  • Zhu H; School of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China.
  • Yang X; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Agricultural University, Zhengzhou, Henan 450046, China.
  • Li W; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China.
  • Du W; Faculty of Veterinary Medicine, Utrecht University, Utrecht 3584CL, the Netherlands.
  • Li Y; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Agricultural University, Zhengzhou, Henan 450046, China. Electronic address: yongtaole@126.com.
  • Li D; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Agricultural University, Zhengzhou, Henan 450046, China. Electronic address: 497222038@sina.com.
Virus Res ; 339: 199275, 2024 01 02.
Article en En | MEDLINE | ID: mdl-38008220
ABSTRACT
The emergence of new coronaviruses poses a significant threat to animal husbandry and human health. Porcine epidemic diarrhea virus (PEDV) is considered a re-emerging porcine enteric coronavirus, which causes fatal watery diarrhea in piglets. Currently, there are no effective drugs to combat PEDV. Drug repurposing screens have emerged as an attractive strategy to accelerate antiviral drug discovery and development. Here, we screened 206 natural products for antiviral activity using live PEDV infection in Vero cells and identified ten candidate antiviral agents. Among them, Tubercidin, a nucleoside analog derived from Streptomyces tubercidicus, showed promising antiviral activity against PEDV infection. Furthermore, we demonstrated that Tubercidin exhibited significant antiviral activity against both classical and variant PEDV. Time of addition assay showed that Tubercidin displayed a significant inhibitory effect on viral post-entry events but not during other periods. Molecular docking analysis indicated that Tubercidin had better docking efficiency and formed hydrophobic interactions with the active pocket of RNA-dependent RNA polymerase (RdRp) of PEDV and other nidoviruses. Additionally, Tubercidin can effectively suppress other porcine nidoviruses, such as SADS-CoV and PRRSV, demonstrating its broad-spectrum antiviral properties. In summary, our findings provide valuable evidence for the antiviral activity of Tubercidin and offer insights into the development of new strategies for the prevention and treatment of coronavirus infections.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Porcinos / Infecciones por Coronavirus / Coronavirus / Nidovirales / Virus de la Diarrea Epidémica Porcina Límite: Animals / Humans Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Porcinos / Infecciones por Coronavirus / Coronavirus / Nidovirales / Virus de la Diarrea Epidémica Porcina Límite: Animals / Humans Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos