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Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review.
Lee, Soo Min; Balakrishnan, Hari Kalathil; Doeven, Egan H; Yuan, Dan; Guijt, Rosanne M.
Afiliación
  • Lee SM; Centre for Regional and Rural Futures (CeRRF), Deakin University, Locked Bag 20000, Geelong, VIC 3220, Australia.
  • Balakrishnan HK; Department of Chemical Engineering, Khalifa University, Abu Dhabi P.O. Box 127788, United Arab Emirates.
  • Doeven EH; School of Life and Environmental Sciences, Deakin University, Locked Bag 20000, Geelong, VIC 3220, Australia.
  • Yuan D; School of Mechanical and Mining Engineering, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Guijt RM; Centre for Regional and Rural Futures (CeRRF), Deakin University, Locked Bag 20000, Geelong, VIC 3220, Australia.
Biosensors (Basel) ; 13(11)2023 Nov 10.
Article en En | MEDLINE | ID: mdl-37998155
Nucleic acid amplification testing facilitates the detection of disease through specific genomic sequences and is attractive for point-of-need testing (PONT); in particular, the early detection of microorganisms can alert early response systems to protect the public and ecosystems from widespread outbreaks of biological threats, including infectious diseases. Prior to nucleic acid amplification and detection, extensive sample preparation techniques are required to free nucleic acids and extract them from the sample matrix. Sample preparation is critical to maximize the sensitivity and reliability of testing. As the enzymatic amplification reactions can be sensitive to inhibitors from the sample, as well as from chemicals used for lysis and extraction, avoiding inhibition is a significant challenge, particularly when minimising liquid handling steps is also desirable for the translation of the assay to a portable format for PONT. The reagents used in sample preparation for nucleic acid testing, covering lysis and NA extraction (binding, washing, and elution), are reviewed with a focus on their suitability for use in PONT.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Nucleicos / Enfermedades Transmisibles Límite: Humans Idioma: En Revista: Biosensors (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Nucleicos / Enfermedades Transmisibles Límite: Humans Idioma: En Revista: Biosensors (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Suiza