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Developmental roles of glomerular epithelial protein-1 in mice molar morphogenesis.
Neupane, Sanjiv; Aryal, Yam Prasad; Kwak, Hee-Jin; Lee, Sung-Gwon; Kim, Tae-Young; Pokharel, Elina; Kim, Ji-Youn; Kim, Jung-Hyeuk; Sohn, Wern-Joo; An, Seo-Young; An, Chang-Hyeon; Jung, Jae-Kwang; Ha, Jung-Hong; Yamamoto, Hitoshi; Cho, Sung-Won; Lee, Sanggyu; Lee, Youngkyun; Park, Kwang-Kyun; Min, Bong-Ki; Park, Chungoo; Kwon, Tae-Yub; Cho, Sung-Jin; Kim, Jae-Young.
Afiliación
  • Neupane S; Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu, Korea.
  • Aryal YP; Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, USA.
  • Kwak HJ; Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu, Korea.
  • Lee SG; School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju, Korea.
  • Kim TY; School of Biological Sciences and Technology, Chonnam National University, Gwangju, Korea.
  • Pokharel E; Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu, Korea.
  • Kim JY; Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu, Korea.
  • Kim JH; Department of Dental Hygiene, Gachon University, Incheon, Korea.
  • Sohn WJ; School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju, Korea.
  • An SY; Pre-Major of Cosmetics and Pharmaceutics, Daegu Haany University, Gyeongsan, Korea.
  • An CH; Department of Oral and Maxillofacial Radiology, School of Dentistry, Kyungpook National University, Daegu, Korea.
  • Jung JK; Department of Oral and Maxillofacial Radiology, School of Dentistry, Kyungpook National University, Daegu, Korea.
  • Ha JH; Department of Oral Medicine, School of Dentistry, Kyungpook National University, Daegu, Korea.
  • Yamamoto H; Department of Conservative Dentistry, School of Dentistry, Kyungpook National University, Daegu, Korea.
  • Cho SW; Department of Histology and Developmental Biology, Tokyo Dental College, Tokyo, Japan.
  • Lee S; Division of Anatomy and Developmental Biology, Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Korea.
  • Lee Y; School of Life Science, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, Korea.
  • Park KK; Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu, Korea.
  • Min BK; Professor Emeritus Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Korea.
  • Park C; Center for Research Facilities, Yeungnam University, Gyeongsan, Korea.
  • Kwon TY; School of Biological Sciences and Technology, Chonnam National University, Gwangju, Korea.
  • Cho SJ; Department of Dental Biomaterials, School of Dentistry, Kyungpook National University, Daegu, Korea.
  • Kim JY; School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju, Korea. sjchobio@chungbuk.ac.kr.
Cell Tissue Res ; 395(1): 53-62, 2024 Jan.
Article en En | MEDLINE | ID: mdl-37985496
Glomerular epithelial protein-1 (Glepp1), a R3 subtype family of receptor-type protein tyrosine phosphatases, plays important role in the activation of Src family kinases and regulates cellular processes such as cell proliferation, differentiation, and apoptosis. In this study, we firstly examined the functional evaluation of Glepp1 in tooth development and morphogenesis. The precise expression level and developmental function of Glepp1 were examined by RT-qPCR, in situ hybridization, and loss and gain of functional study using a range of in vitro organ cultivation methods. Expression of Glepp1 was detected in the developing tooth germs in cap and bell stage of tooth development. Knocking down Glepp1 at E13 for 2 days showed the altered expression levels of tooth development-related signaling molecules, including Bmps, Dspp, Fgf4, Lef1, and Shh. Moreover, transient knock down of Glepp1 revealed alterations in cellular physiology, examined by the localization patterns of Ki67 and E-cadherin. Similarly, knocking down of Glepp1 showed disrupted enamel rod and interrod formation in 3-week renal transplanted teeth. In addition, due to attrition of odontoblastic layers, the expression signals of Dspp and the localization of NESTIN were almost not detected after knock down of Glepp1; however, their expressions were increased after Glepp1 overexpression. Thus, our results suggested that Glepp1 plays modulating roles during odontogenesis by regulating the expression levels of signaling molecules and cellular events to achieve the proper structural formation of hard tissue matrices in mice molar development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diente / Proteínas Tirosina Fosfatasas Clase 3 Similares a Receptores Límite: Animals Idioma: En Revista: Cell Tissue Res Año: 2024 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diente / Proteínas Tirosina Fosfatasas Clase 3 Similares a Receptores Límite: Animals Idioma: En Revista: Cell Tissue Res Año: 2024 Tipo del documento: Article Pais de publicación: Alemania