Your browser doesn't support javascript.
loading
Quality by design endorsed atorvastatin-loaded nanostructured lipid carriers embedded in pH-responsive gel for melanoma.
Bagasariya, Deepkumar; Charankumar, Kondasingh; Shah, Saurabh; Famta, Paras; Fernandes, Valencia; Shahrukh, Syed; Khatri, Dharmendra Kumar; Singh, Shashi Bala; Srivastava, Saurabh.
Afiliación
  • Bagasariya D; Department of Pharmaceutics, Pharmaceutical Innovation and Translational Research Lab (PITRL), National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, India.
  • Charankumar K; Department of Pharmaceutics, Pharmaceutical Innovation and Translational Research Lab (PITRL), National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, India.
  • Shah S; Department of Pharmaceutics, Pharmaceutical Innovation and Translational Research Lab (PITRL), National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, India.
  • Famta P; Department of Pharmaceutics, Pharmaceutical Innovation and Translational Research Lab (PITRL), National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, India.
  • Fernandes V; Department of Biological Sciences, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, India.
  • Shahrukh S; Department of Pharmaceutics, Pharmaceutical Innovation and Translational Research Lab (PITRL), National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, India.
  • Khatri DK; Department of Biological Sciences, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, India.
  • Singh SB; Department of Biological Sciences, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, India.
  • Srivastava S; Department of Pharmaceutics, Pharmaceutical Innovation and Translational Research Lab (PITRL), National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, India.
J Microencapsul ; 41(1): 27-44, 2024 Jan.
Article en En | MEDLINE | ID: mdl-37982590
AIM: Our aim was to repurpose atorvastatin for melanoma by encapsulating in a nanostructured lipid carrier matrix to promote tumour cell internalisation and skin permeation. pH-responsive chitosan gel was employed to restrict At-NLCs in upper dermal layers. METHODS: We utilised a quality by design approach for encapsulating At within the NLC matrix. Further, cellular uptake and cytotoxicity was evaluated along with pH-responsive release and ex vivo skin permeation. RESULTS: Cytotoxicity assay showed 3.13-fold enhanced cytotoxicity on melanoma cells compared to plain drug with nuclear staining showing apoptotic markers. In vitro, release studies showed 5.9-fold rapid release in chitosan gel matrix at pH 5.5 compared to neutral pH. CONCLUSIONS: At-NLCs prevented precipitation, promoted skin permeation, and SK-MEL 28 cell internalisation. The localisation of NLCs on the upper dermal layer due to electrostatic interactions of skin with chitosan gel diminished the incidence of untoward systemic effects.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitosano / Nanoestructuras / Melanoma Límite: Humans Idioma: En Revista: J Microencapsul Asunto de la revista: FARMACIA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitosano / Nanoestructuras / Melanoma Límite: Humans Idioma: En Revista: J Microencapsul Asunto de la revista: FARMACIA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido