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Engineered bacteria titrate hydrogen sulfide and induce concentration-dependent effects on the host in a gut microphysiological system.
Hayes, Justin A; Lunger, Anna W; Sharma, Aayushi S; Fernez, Matthew T; Carrier, Rebecca L; Koppes, Abigail N; Koppes, Ryan; Woolston, Benjamin M.
Afiliación
  • Hayes JA; Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
  • Lunger AW; Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
  • Sharma AS; Department of Mechanical and Industrial Engineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA; Department of Bioengineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
  • Fernez MT; Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
  • Carrier RL; Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA; Department of Bioengineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA; Department of Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA
  • Koppes AN; Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA; Department of Bioengineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
  • Koppes R; Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA. Electronic address: r.koppes@northeastern.edu.
  • Woolston BM; Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA. Electronic address: b.woolston@northeastern.edu.
Cell Rep ; 42(12): 113481, 2023 12 26.
Article en En | MEDLINE | ID: mdl-37980564
Hydrogen sulfide (H2S) is a gaseous microbial metabolite whose role in gut diseases is debated, with contradictory results stemming from experimental difficulties associated with accurate dosing and measuring H2S and the use of model systems that do not accurately represent the human gut environment. Here, we engineer Escherichia coli to titrate H2S across the physiological range in a gut microphysiological system (chip) supportive of the co-culture of microbes and host cells. The chip is engineered to maintain H2S gas tension and enables visualization of co-culture in real time with confocal microscopy. Engineered strains colonize the chip and are metabolically active for 2 days, during which they produce H2S across a 16-fold range and induce changes in host gene expression and metabolism in an H2S-concentration-dependent manner. These results validate a platform for studying the mechanisms underlying microbe-host interactions by enabling experiments that are infeasible with current animal and in vitro models.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbioma Gastrointestinal / Sulfuro de Hidrógeno Límite: Animals / Humans Idioma: En Revista: Cell Rep Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbioma Gastrointestinal / Sulfuro de Hidrógeno Límite: Animals / Humans Idioma: En Revista: Cell Rep Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos