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Immobilizing glucose oxidase on AuCu hydrogels for enhanced electrochromic biosensing.
Chen, Yanan; Jiao, Lei; Li, Ruimin; Hu, Lijun; Jia, Xiangkun; Zhu, Zhijun; Zhai, Yanling; Lu, Xiaoquan.
Afiliación
  • Chen Y; Institute of Molecular Metrology, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, PR China.
  • Jiao L; Institute of Molecular Metrology, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, PR China. Electronic address: jiaolei@qdu.edu.cn.
  • Li R; Institute of Molecular Metrology, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, PR China.
  • Hu L; Institute of Molecular Metrology, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, PR China.
  • Jia X; Institute of Molecular Metrology, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, PR China.
  • Zhu Z; Institute of Hybrid Materials, National Center of International Research for Hybrid Materials Technology, National Base of International Science & Technology Cooperation, College of Materials Science and Engineering, Qingdao University, Qingdao, 266071, PR China. Electronic address: zhuzhijun@qd
  • Zhai Y; Institute of Molecular Metrology, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, PR China. Electronic address: zhaiyanling@qdu.edu.cn.
  • Lu X; Institute of Molecular Metrology, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, PR China. Electronic address: luxq@nwnu.edu.cn.
Anal Chim Acta ; 1283: 341977, 2023 Dec 01.
Article en En | MEDLINE | ID: mdl-37977794
Development of highly sensitive and accurate biosensors still faces a great challenge. Herein, glucose oxidase (GOx) is efficiently immobilized on the AuCu hydrogels owing to their porous structure and interfacial interaction, demonstrating enhanced catalytic activity, satisfactory stability and recyclability. Besides, by integration of AuCu@GOx and electrochromic material of Prussian blue, a sensitive and stable biosensing platform based on the excellent electrochromic property of Prussian blue and the enhanced enzyme activity of AuCu@GOx is developed, which enables the electrochemical and visual dual-mode detection of glucose. The as-constructed biosensing platform possesses a wide linear range, and good selectivity for glucose detection with a limit of detection of 0.82 µM in visual mode and 0.84 µM in electrochemical mode. This easy-to-operate biosensing platform opens a door for the practical application of the multi-mode strategy for glucose detection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Glucosa Oxidasa Idioma: En Revista: Anal Chim Acta Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Glucosa Oxidasa Idioma: En Revista: Anal Chim Acta Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos