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Functional maturation of the rod bipolar to AII-amacrine cell ribbon synapse in the mouse retina.
Kim, Mean-Hwan; Strazza, Paulo; Puthussery, Teresa; Gross, Owen P; Taylor, W Rowland; von Gersdorff, Henrique.
Afiliación
  • Kim MH; The Vollum Institute, Oregon Health & Science University, Portland, OR 97239, USA; Allen Institute for Brain Science, Seattle, WA 98109, USA. Electronic address: meanhwan@gmail.com.
  • Strazza P; The Vollum Institute, Oregon Health & Science University, Portland, OR 97239, USA.
  • Puthussery T; Casey Eye Institute, Oregon Health & Science University, Portland, OR 97239, USA; Herbert Wertheim School of Optometry & Vision Science, Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Gross OP; The Vollum Institute, Oregon Health & Science University, Portland, OR 97239, USA; Department of Physics, Reed College, Portland, OR 97202, USA.
  • Taylor WR; Casey Eye Institute, Oregon Health & Science University, Portland, OR 97239, USA; Herbert Wertheim School of Optometry & Vision Science, Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
  • von Gersdorff H; The Vollum Institute, Oregon Health & Science University, Portland, OR 97239, USA; Casey Eye Institute, Oregon Health & Science University, Portland, OR 97239, USA. Electronic address: vongersd@ohsu.edu.
Cell Rep ; 42(11): 113440, 2023 11 28.
Article en En | MEDLINE | ID: mdl-37976158
Retinal ribbon synapses undergo functional changes after eye opening that remain uncharacterized. Using light-flash stimulation and paired patch-clamp recordings, we examined the maturation of the ribbon synapse between rod bipolar cells (RBCs) and AII-amacrine cells (AII-ACs) after eye opening (postnatal day 14) in the mouse retina at near physiological temperatures. We find that light-evoked excitatory postsynaptic currents (EPSCs) in AII-ACs exhibit a slow sustained component that increases in magnitude with advancing age, whereas a fast transient component remains unchanged. Similarly, paired recordings reveal a dual-component EPSC with a slower sustained component that increases during development, even though the miniature EPSC (mEPSC) amplitude and kinetics do not change significantly. We thus propose that the readily releasable pool of vesicles from RBCs increases after eye opening, and we estimate that a short light flash can evoke the release of ∼4,000 vesicles onto a single mature AII-AC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sinapsis / Células Amacrinas Límite: Animals Idioma: En Revista: Cell Rep Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sinapsis / Células Amacrinas Límite: Animals Idioma: En Revista: Cell Rep Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos