Your browser doesn't support javascript.
loading
Nanoplastics from ground polyethylene terephthalate food containers: Genotoxicity in human lung epithelial A549 cells.
Alzaben, Mohammad; Burve, Regina; Loeschner, Katrin; Møller, Peter; Roursgaard, Martin.
Afiliación
  • Alzaben M; Department of Public Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5A, DK-1014 Copenhagen K, Denmark.
  • Burve R; Department of Energy Conversion and Storage, Technical University of Denmark, Anker Engelunds Vej 301, DK-2800, Lyngby, Denmark.
  • Loeschner K; National Food Institute, Technical University of Denmark, Kemitorvet 201, DK-2800, Lyngby, Denmark.
  • Møller P; Department of Public Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5A, DK-1014 Copenhagen K, Denmark.
  • Roursgaard M; Department of Public Health, Section of Environmental Health, University of Copenhagen, Øster Farimagsgade 5A, DK-1014 Copenhagen K, Denmark. Electronic address: mwro@sund.ku.dk.
Mutat Res Genet Toxicol Environ Mutagen ; 892: 503705, 2023.
Article en En | MEDLINE | ID: mdl-37973296
The ubiquitous pollution of plastic particles in most environmental matrices leads to concern about any potential adverse effects on human health. Most studies on the toxicological effect of nanoplastics has focused on standard particles of polystyrene. In reality humans are exposed to a large variety of different types and sizes of plastic material via oral intake and inhalation. In this study, we investigated the effect of polyethylene terephthalate (PET) nanoplastic particles from ground food containers from a supermarket. The aim was to investigate a possible link between exposure to PET nanoplastics and genotoxic response in a cell model of the human airway epithelial (A549) cells. Further, we investigated the combined effect of PET and chemicals known to alter the cellular redox state, as a model of partially compromised antioxidant defense system. DNA damage was assessed by the alkaline comet assay. The ground PET nanoplastics have a mean hydrodynamic diameter of 136 nm in water. The results showed that PET exposure led to increased reactive oxygen species production (approximately 30 % increase compared to unexposed cells). In addition, exposure to PET nanoplastic increased the level of DNA strand breaks (net increase = 0.10 lesions/106 base pair, 95 % confidence interval: 0.01, 0.18 lesions/106 base pair). Pre- or post-exposure to hydrogen peroxide or buthionine sulfoximine did not lead to a higher level of DNA damage. Overall, the study shows that exposure to PET nanoplastics increases both intracellular reactive oxygen production and DNA damage in A549 cells.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tereftalatos Polietilenos / Microplásticos Límite: Humans Idioma: En Revista: Mutat Res Genet Toxicol Environ Mutagen Año: 2023 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tereftalatos Polietilenos / Microplásticos Límite: Humans Idioma: En Revista: Mutat Res Genet Toxicol Environ Mutagen Año: 2023 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Países Bajos