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Expression of Galectin-9-related immune checkpoint receptors in B-cell acute lymphoblastic leukemia.
Akbar, Armin; Asgarian-Omran, Hossein; Valadan, Reza; Dindarloo, Mohammad-Mehdi; Najafi, Ahmad; Kahrizi, Amir; Poursheikhani, Arash; Karami, Hossein; Naderi, Mohammad; Sabeti, Shayan; Tehrani, Mohsen.
Afiliación
  • Akbar A; Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Asgarian-Omran H; Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Valadan R; Molecular and Cell-Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
  • Dindarloo MM; Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Najafi A; Molecular and Cell-Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
  • Kahrizi A; Department of Biostatistics and Epidemiology, Mazandaran University of Medical Sciences, Sari, Iran.
  • Poursheikhani A; Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Karami H; Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Naderi M; Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran.
  • Sabeti S; Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Tehrani M; Department of Hematology and Oncology, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, Iran.
Iran J Basic Med Sci ; 26(12): 1468-1474, 2023.
Article en En | MEDLINE | ID: mdl-37970435
Objectives: Exhausted CD8+ T-cells over-express immune checkpoint receptors (ICRs), which interact with their ligands on malignant cells. However, some ICRs have been reported to be expressed on both T-cells and tumor cells, including V-domain immunoglobulin suppressor of T cell activation (VISTA), Galectin-9, and T-cell immunoglobulin mucin-3 (TIM-3). We aimed to evaluate the mRNA expression of VISTA, Galectin-9, and TIM-3 on CD8+ T-cells and leukemic cells in B-cell acute lymphoblastic leukemia (B-ALL). Materials and Methods: Samples were obtained from 26 untreated B-ALL patients and 25 control subjects. CD8+ T-cells were isolated using Magnetic Activated Cell Sorting (MACS). Relative gene expression was then evaluated by qRT-PCR with specific primers for VISTA, Galectin-9, and TIM-3. Also, the mRNA expression profile and clinical data of 154 B-ALL patients were obtained from the TARGET. Results: mRNA expression of Galectin-9 on CD8+ T-cells in B-ALL patients was significantly lower than those in the control group (P=0.043), while VISTA expression was not significantly different between the two study groups (P=0.259). Besides, TIM-3 expression was significantly higher in B-ALL patients than in the control group (P<0.001). Also, data obtained from TARGET showed that the relapse incidence was not significantly different between patients with high and low expression of Galectin-9 and TIM-3 in leukemic cells (P=0.360 and P=0.655, respectively). Conclusion: Collectively, gene expression results suggest an important role for TIM-3, but not VISTA and Galectin-9, in B-ALL and it seems that TIM-3 could be a candidate for immune checkpoint therapy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Iran J Basic Med Sci Año: 2023 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Irán
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Iran J Basic Med Sci Año: 2023 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Irán