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Exploring Psoriasis Inflammatory Microenvironment by NanoString Technologies.
Ricci, Alessia Andrea; Dapavo, Paolo; Mastorino, Luca; Roccuzzo, Gabriele; Wolff, Samanta; Ribero, Simone; Cassoni, Paola; Senetta, Rebecca; Quaglino, Pietro.
Afiliación
  • Ricci AA; Pathology Unit, Department of Medical Sciences, University of Turin, 10126 Turin, Italy.
  • Dapavo P; Department of Medical Sciences, Section of Dermatology, University of Turin, 10126 Turin, Italy.
  • Mastorino L; Department of Medical Sciences, Section of Dermatology, University of Turin, 10126 Turin, Italy.
  • Roccuzzo G; Department of Medical Sciences, Section of Dermatology, University of Turin, 10126 Turin, Italy.
  • Wolff S; Department of Medical Sciences, Section of Dermatology, University of Turin, 10126 Turin, Italy.
  • Ribero S; Department of Medical Sciences, Section of Dermatology, University of Turin, 10126 Turin, Italy.
  • Cassoni P; Pathology Unit, Department of Medical Sciences, University of Turin, 10126 Turin, Italy.
  • Senetta R; Pathology Unit, Department of Oncology, University of Turin, 10126 Turin, Italy.
  • Quaglino P; Department of Medical Sciences, Section of Dermatology, University of Turin, 10126 Turin, Italy.
J Clin Med ; 12(21)2023 Oct 28.
Article en En | MEDLINE | ID: mdl-37959285
Psoriasis is a chronic inflammatory skin disease whose molecular mechanisms and microenvironment are poorly understood. We performed gene expression analysis through the nCounter® PanCancer Immune Profiling Panel (NanoString Technologies, Seattle, WA, USA) on 22 FFPE punch biopsies from 19 psoriasis-affected patients. A subset of five cases was analyzed before (T0) and after 6 months (T6) of treatment with dimethyl fumarate (DMF) to address immune microenvironment changes. Molecular comparisons according to biopsy site and age of onset showed a different distribution of innate immune cells (mast cells, macrophages, NK cells, and DC cells) and pathways (complement regulation and transporter functions). The analysis according to PASI (Psoriasis Area and Severity Index) led to non-significant results, suggesting no link between molecular expression profile and clinical amount of skin disease. In DMF-treated patients, we observed a strong immunomodulatory effect after treatment: A subversion of exhausted CD8 T cells, NK CD56dim cells, Tregs, neutrophils, CD45+ cells, T cells, B cells, and macrophages was reported between the two analyzed time-points, as well as the reduction in pro-inflammatory pathways and molecules, including cytotoxicity, pathogen defense, antigen processing, adhesion, cell cycle, chemokines, cytokines, and interleukins. The inflammatory psoriatic microenvironment can be modulated using DMF with encouraging results, achieving an immune-tolerant and non-inflammatory condition through the regulation of both innate and adaptive immunity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Clin Med Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Clin Med Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza