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Identification and characterization of a highly virulent Citrobacter freundii isolate and its activation on immune responses in largemouth bass (Micropterus salmoides).
Gong, Cuiping; Guo, Mengya; Lou, Jianfeng; Zhang, Liwen; An, Zhenhua; Vakharia, Vikram N; Kong, Weiguang; Liu, Xiaodan.
Afiliación
  • Gong C; Huzhou Academy of Agricultural Sciences, Huzhou, 313000, China.
  • Guo M; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
  • Lou J; Huzhou Nanxun District Agricultural Technology Extension Service Center, Huzhou, 313000, China.
  • Zhang L; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
  • An Z; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
  • Vakharia VN; Institute of Marine and Environmental Technology, University of Maryland Baltimore Country, Baltimore, MD, 21202, USA.
  • Kong W; Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. Electronic address: kongweiguang@ihb.ac.cn.
  • Liu X; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou, China. Electronic
Fish Shellfish Immunol ; 143: 109224, 2023 Dec.
Article en En | MEDLINE | ID: mdl-37956797
Citrobacter freundii, a common pathogen of freshwater fish, causes significant commercial losses to the global fish farming industry. In the present study, a highly pathogenic C. freundii strain was isolated and identified from largemouth bass (Micropterus salmoides). The pathogenicity and antibiotic sensitivity of the C. freundii strain were evaluated, and the histopathology and host immune response of largemouth bass infected with C. freundii were investigated. The results showed that C. freundii was the pathogen causing disease outbreaks in largemouth bass, and the infected fish showed typical signs of acute hemorrhages and visceral enlargement. Antimicrobial susceptibility testing showed that the C. freundii strain was resistant to Kanamycin, Medimycin, Clindamycin, Penicillin, Oxacillin, Ampicillin, Cephalexin, Cefazolin, Cefradine and Vancomycin. Histopathological analysis showed different pathological changes in major tissues of diseased fish. In addition, humoral immune factors such as superoxide dismutase (SOD), catalase (CAT) and lysozyme (LZM) were used as serum indicators to evaluate the immune response of largemouth bass after infection. Quantitative real-time PCR (qRT-PCR) was performed to investigate the expression pattern of immune-related genes (CXCR1, IL-8, IRF7, IgM, CD40, IFN-γ, IL-1ß, Hep1, and Hep2) in liver, spleen, and head kidney tissues, which demonstrated a strong immune response induced by C. freundii infection in largemouth bass. The present study provides insights into the pathogenic mechanism of C. freundii and immune response in largemouth bass, promoting the prevention and treatment of diseases caused by C. freundii infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lubina / Enfermedades de los Peces Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lubina / Enfermedades de los Peces Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido