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Bovine herpesvirus-1 gE protein inhibits IFN-ß production to enhance replication by promoting MAVS ubiquitination and interfering with the interaction between IRF3 and CBP/p300.
Liu, Yang; Cui, Jin; Kang, Jingli; Wang, Zhiliang; Xu, Xingang; Wu, Faxing.
Afiliación
  • Liu Y; Key Laboratory of Animal Biosafety Risk Prevention and Control of Ministry of Agriculture and Rural Affairs (South), China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • Cui J; Key Laboratory of Animal Biosafety Risk Prevention and Control of Ministry of Agriculture and Rural Affairs (South), China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China.
  • Kang J; Key Laboratory of Animal Biosafety Risk Prevention and Control of Ministry of Agriculture and Rural Affairs (South), China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China.
  • Wang Z; Key Laboratory of Animal Biosafety Risk Prevention and Control of Ministry of Agriculture and Rural Affairs (South), China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China.
  • Xu X; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: tiger2003@nwsuaf.edu.cn.
  • Wu F; Key Laboratory of Animal Biosafety Risk Prevention and Control of Ministry of Agriculture and Rural Affairs (South), China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China. Electronic address: wufaxing@cahec.cn.
Vet Microbiol ; 287: 109899, 2023 Dec.
Article en En | MEDLINE | ID: mdl-37931576
Bovine herpesvirus-1 (BoHV-1) can infect all breeds of cattle and cause respiratory and genital tract diseases. In the process of viral infection, viruses can use their own proteins to suppress the innate immunity of the host and promote its replication; however, the mechanism by which BoHV-1 evades the innate immune response is not fully understood. In this study, we found that rabbits inoculated with the live gene deletion vaccine BoHV-1-△gI/gE/TK generated higher interferon-ß (IFN-ß) production in the serum, liver, lung and kidney than rabbits inoculated with wt BoHV-1, which led to milder lesions in the lung and kidney. We performed gene deletion and ectopic expression experiments on viral proteins and found that gE was the major protein that inhibited IFN-ß expression. Further studies showed that MAVS and IRF3 were the targets of gE, and the specific mechanism was that gE inhibited IFN-ß production by promoting MAVS ubiquitination and interfering with the interaction between IRF3 and CBP/p300. These results suggest a new way of BoHV-1 inhibition of IFN-ß production to evade the host innate immunity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Herpesvirus Bovino 1 Límite: Animals Idioma: En Revista: Vet Microbiol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Herpesvirus Bovino 1 Límite: Animals Idioma: En Revista: Vet Microbiol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos