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A New Player in the Mechanobiology of Deep Fascia: Yes-Associated Protein (YAP).
Pirri, Carmelo; Caroccia, Brasilina; Angelini, Andrea; Piazza, Maria; Petrelli, Lucia; Caputo, Ilaria; Montemurro, Chiara; Ruggieri, Pietro; De Caro, Raffaele; Stecco, Carla.
Afiliación
  • Pirri C; Department of Neurosciences, Institute of Human Anatomy, University of Padova, 35121 Padova, Italy.
  • Caroccia B; Department of Medicine-DIMED, University of Padova, 35128 Padova, Italy.
  • Angelini A; Department of Orthopedics and Orthopedic Oncology, University of Padova, 35128 Padova, Italy.
  • Piazza M; Department of Medicine-DIMED, University of Padova, 35128 Padova, Italy.
  • Petrelli L; Department of Medicine-DIMED, University of Padova, 35128 Padova, Italy.
  • Caputo I; Department of Medicine-DIMED, University of Padova, 35128 Padova, Italy.
  • Montemurro C; Independent Researcher, ty Padova, 35120 Padova, Italy.
  • Ruggieri P; Department of Orthopedics and Orthopedic Oncology, University of Padova, 35128 Padova, Italy.
  • De Caro R; Department of Neurosciences, Institute of Human Anatomy, University of Padova, 35121 Padova, Italy.
  • Stecco C; Department of Neurosciences, Institute of Human Anatomy, University of Padova, 35121 Padova, Italy.
Int J Mol Sci ; 24(20)2023 Oct 20.
Article en En | MEDLINE | ID: mdl-37895068
Recent studies have demonstrated that fascial fibroblasts are susceptible to mechanical stimuli, leading to the remodeling of the extracellular matrix (ECM). Moreover, the extensive literature on Yes-associated protein (YAP) has shown its role in cell mechanics, linking cell properties, such as shape, adhesion, and size, to the expression of specific genes. The aim of this study was to investigate the presence of YAP in deep fascia and its activation after a mechanical stimulus was induced via a focal extracorporeal shockwave (fESW) treatment. Thoracolumbar fascia (TLF) samples were collected from eight patients (age: 30-70 years; four males and four females) who had undergone spine elective surgical procedures at the Orthopedic Clinic of University of Padova. YAP was measured in both tissue and TLF-derived fibroblasts through immunoblotting. COL1A1 and HABP2 gene expression were also evaluated in fibroblasts 2, 24, and 48 h after the fESW treatment. YAP was expressed in all the examined tissues. The ratio between the active/inactive forms (YAP/p-YAP) of the protein significantly increased in fascial fibroblasts after mechanical stimulation compared to untreated cells (p = 0.0022). Furthermore, COL1A1 and HABP2 gene expression levels were increased upon treatment. These findings demonstrate that YAP is expressed in the deep fascia of the thoracolumbar region, suggesting its involvement in fascial mechanotransduction processes, remodeling, regeneration, and fibrogenesis. This study indicates, for the first time, that YAP is a "new player" in the mechanobiology of deep fascia.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mecanotransducción Celular / Proteínas Señalizadoras YAP Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mecanotransducción Celular / Proteínas Señalizadoras YAP Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza