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Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging.
Nasu, Yusuke; Aggarwal, Abhi; Le, Giang N T; Vo, Camilla Trang; Kambe, Yuki; Wang, Xinxing; Beinlich, Felix R M; Lee, Ashley Bomin; Ram, Tina R; Wang, Fangying; Gorzo, Kelsea A; Kamijo, Yuki; Boisvert, Marc; Nishinami, Suguru; Kawamura, Genki; Ozawa, Takeaki; Toda, Hirofumi; Gordon, Grant R; Ge, Shaoyu; Hirase, Hajime; Nedergaard, Maiken; Paquet, Marie-Eve; Drobizhev, Mikhail; Podgorski, Kaspar; Campbell, Robert E.
Afiliación
  • Nasu Y; Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan. nasu@chem.s.u-tokyo.ac.jp.
  • Aggarwal A; PRESTO, Japan Science and Technology Agency, Chiyoda-ku, Tokyo, 102-0075, Japan. nasu@chem.s.u-tokyo.ac.jp.
  • Le GNT; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.
  • Vo CT; Allen Institute for Neural Dynamics, Seattle, WA, 98109, USA.
  • Kambe Y; Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
  • Wang X; Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada.
  • Beinlich FRM; Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, 2200, Denmark.
  • Lee AB; Department of Pharmacology, Graduate School of Medical and Dental Science, Kagoshima University, Sakuragaoka, Kagoshima, 890-8544, Japan.
  • Ram TR; Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, NY, 11794, USA.
  • Wang F; Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, 2200, Denmark.
  • Gorzo KA; Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, 2200, Denmark.
  • Kamijo Y; Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.
  • Boisvert M; Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.
  • Nishinami S; Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.
  • Kawamura G; Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
  • Ozawa T; CERVO Brain Research Centre, Québec, QC, G1J 2G3, Canada.
  • Toda H; Department of Biochemistry, Microbiology and Bioinformatics, Laval University, Québec, QC, G1E 1T2, Canada.
  • Gordon GR; International Institute for Integrative Sleep Medicine, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan.
  • Ge S; Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
  • Hirase H; Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
  • Nedergaard M; International Institute for Integrative Sleep Medicine, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan.
  • Paquet ME; Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.
  • Drobizhev M; Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, NY, 11794, USA.
  • Podgorski K; Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, 2200, Denmark.
  • Campbell RE; Center for Translational Neuromedicine, University of Rochester Medical Center, Rochester, NY, 14642, USA.
Nat Commun ; 14(1): 6598, 2023 10 27.
Article en En | MEDLINE | ID: mdl-37891202
L-Lactate is increasingly appreciated as a key metabolite and signaling molecule in mammals. However, investigations of the inter- and intra-cellular dynamics of L-lactate are currently hampered by the limited selection and performance of L-lactate-specific genetically encoded biosensors. Here we now report a spectrally and functionally orthogonal pair of high-performance genetically encoded biosensors: a green fluorescent extracellular L-lactate biosensor, designated eLACCO2.1, and a red fluorescent intracellular L-lactate biosensor, designated R-iLACCO1. eLACCO2.1 exhibits excellent membrane localization and robust fluorescence response. To the best of our knowledge, R-iLACCO1 and its affinity variants exhibit larger fluorescence responses than any previously reported intracellular L-lactate biosensor. We demonstrate spectrally and spatially multiplexed imaging of L-lactate dynamics by coexpression of eLACCO2.1 and R-iLACCO1 in cultured cells, and in vivo imaging of extracellular and intracellular L-lactate dynamics in mice.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Ácido Láctico Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Ácido Láctico Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido