Your browser doesn't support javascript.
loading
Plasma Treatment of PDMS for Microcontact Printing (µCP) of Lectins Decreases Silicone Transfer and Increases the Adhesion of Bladder Cancer Cells.
Zemla, Joanna; Szydlak, Renata; Gajos, Katarzyna; Kozlowski, Lukasz; Zielinski, Tomasz; Luty, Marcin; Øvreeide, Ingrid H; Prot, Victorien E; Stokke, Bjørn T; Lekka, Malgorzata.
Afiliación
  • Zemla J; Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland.
  • Szydlak R; Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland.
  • Gajos K; M. Smoluchowski Institute of Physics, Jagiellonian University, 30348 Kraków, Poland.
  • Kozlowski L; Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland.
  • Zielinski T; Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland.
  • Luty M; Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland.
  • Øvreeide IH; Biophysics and Medical Technology, Department of Physics, The Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway.
  • Prot VE; Biomechanics, Department of Structural Engineering, The Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway.
  • Stokke BT; Biophysics and Medical Technology, Department of Physics, The Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway.
  • Lekka M; Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland.
ACS Appl Mater Interfaces ; 2023 Oct 27.
Article en En | MEDLINE | ID: mdl-37889219
The present study investigates silicone transfer occurring during microcontact printing (µCP) of lectins with polydimethylsiloxane (PDMS) stamps and its impact on the adhesion of cells. Static adhesion assays and single-cell force spectroscopy (SCFS) are used to compare adhesion of nonmalignant (HCV29) and cancer (HT1376) bladder cells, respectively, to high-affinity lectin layers (PHA-L and WGA, respectively) prepared by physical adsorption and µCP. The chemical composition of the µCP lectin patterns was monitored by time-of-flight secondary ion mass spectrometry (ToF-SIMS). We show that the amount of transferred silicone in the µCP process depends on the preprocessing of the PDMS stamps. It is revealed that silicone contamination within the patterned lectin layers inhibits the adhesion of bladder cells, and the work of adhesion is lower for µCP lectins than for drop-cast lectins. The binding capacity of microcontact printed lectins was larger when the PDMS stamps were treated with UV ozone plasma as compared to sonication in ethanol and deionized water. ToF-SIMS data show that ozone-based treatment of PDMS stamps used for µCP of lectin reduces the silicone contamination in the imprinting protocol regardless of stamp geometry (flat vs microstructured). The role of other possible contributors, such as the lectin conformation and organization of lectin layers, is also discussed.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2023 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2023 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Estados Unidos