Objective: The objective of this study was to investigate the preventive and therapeutic effects of Shenzhu Jiedu Granule on COVID-19 using network pharmacology and animal experiments. Methods: Obtain the chemical components of Shenshu Jiedu Granule from the online pharmacology database and analysis platform (ETCM) of the Chinese traditional medicine system, obtain the potential target of the compound through the UniProt database, and obtain the related target of COVID-19 from GeneCards and OMIM databases; Construct a component target network diagram using Cytascape 3.7.0 software, import the protein interaction (PPI) of intersection targets into Cytascape software through STRING database, and use the Metascape platform to conduct gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomics (KEGG) enrichment analysis on intersection targets.To explore its anti-inflammatory and analgesic effects through animal ear swelling, hot plate and torsion experiments. Results: Analysis revealed 72 key target proteins associated with the effects of Shenzhu Jiedu Granule demonstrated that mainly interleukin-6 (IL-6), interleukin-1 ß (IL 1 ß), B cells κ Light peptide gene enhancer nuclear factor inhibitor 1 (NFKB1), B cells κ Light peptide gene enhancer nuclear factor inhibitor 1B (NFKB1A), interferon ß IFNB1, tumor necrosis factor TNF, recombinant human mitogen activated protein kinase 12 (MAPK12), serine/threonine kinase 1 (AKT1), B cells κ Light peptide gene enhancer inhibitor kinase ß (IKBKB), etc.The analysis found that it is mainly related to multiple biological processes such as intercellular immune regulation, inflammatory cytokines, and ion channels in the microenvironment; KEGG analysis showed that COVID-19 pathway, influenza virus pathway and multiple immune inflammatory response pathways were mainly involved. Obtained 91 effective ingredients of Shenshu Jiedu Granule, 10 anti-inflammatory, bactericidal, and antiviral compounds, and 4 immune enhancing compounds. Shenzhu Jiedu Granule demonstrated an inhibitory effect on xylene-induced ear swelling in mice and significantly enhanced the anti-inflammatory and analgesic effects by reducing body twists and prolonging the time mice licked their feet. Conclusions: It is suggested that Shenzhu Jiedu Granule has anti COVID-19, influenza virus, antibacterial and anti-inflammatory effects, and can significantly enhance the anti-inflammatory and analgesic effects of mice, which highlight the significance of the study in the context of current global health concerns.