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Mononuclear cells from patients with rhupus are influenced by TNF in their production of gp130/sIL-6Rb and APRIL.
Amezcua-Guerra, Luis M; Sánchez-Muñoz, Fausto; Guzmán-García, Santiago; Márquez-Velasco, Ricardo; Becerril-Villanueva, Enrique; Vázquez-Panchos, Yadira; Juárez-Vicuña, Yaneli.
Afiliación
  • Amezcua-Guerra LM; Department of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
  • Sánchez-Muñoz F; Department of Health Care, Universidad Autónoma Metropolitana-Xochimilco, Mexico City, Mexico.
  • Guzmán-García S; Department of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
  • Márquez-Velasco R; Department of Rheumatology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
  • Becerril-Villanueva E; Department of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
  • Vázquez-Panchos Y; Laboratory of Psychoimmunology, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Mexico City, Mexico.
  • Juárez-Vicuña Y; Department of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
Lupus Sci Med ; 10(2)2023 10.
Article en En | MEDLINE | ID: mdl-37880158
OBJECTIVE: Rhupus is a rare disease that shares characteristics of systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). While several studies have explored the clinical and immunological profiles of patients with rhupus, the underlying cause of the disease remains unknown due to its complex pathogenesis. The objective of this study was to investigate the role of tumour necrosis factor (TNF) in the production of inflammatory molecules by peripheral blood mononuclear cells (PBMCs) from patients with rhupus. METHODS: The study involved five healthy controls, seven patients with rhupus and seven patients with SLE. PBMCs were obtained from each participant and stimulated with recombinant human TNF for 24 hours. The levels of various molecules secreted by the cells, such as cytokines and chemokines, were measured using immunobead-based assays on xMAP technology. RESULTS: The production levels of some molecules were higher in TNF-stimulated PBMCs from patients with rhupus and SLE than in unstimulated cells. In addition, the levels of certain molecules, including gp130/sIL-6Rb, a proliferation-inducing ligand (APRIL), interferon-ß, matrix metalloproteinase-3 and interleukin (IL)-12, were higher in PBMCs from patients with rhupus even without TNF stimulation. Similarly, the levels of gp130/sIL-6Rb and APRIL were higher in TNF-stimulated PBMCs from patients with rhupus than in healthy controls. These results were further validated against patients with RA using enzyme-linked immunosorbent assay. CONCLUSIONS: These findings suggest that the spontaneous production of molecules by cells from patients with rhupus may contribute to the development of the disease, and that TNF may play a role in this process by regulating the secretion of gp130/sIL-6Rb and APRIL.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artritis Reumatoide / Lupus Eritematoso Sistémico Límite: Humans Idioma: En Revista: Lupus Sci Med Año: 2023 Tipo del documento: Article País de afiliación: México Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artritis Reumatoide / Lupus Eritematoso Sistémico Límite: Humans Idioma: En Revista: Lupus Sci Med Año: 2023 Tipo del documento: Article País de afiliación: México Pais de publicación: Reino Unido