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Low doses of IFN-γ maintain self-renewal of leukemia stem cells in acute myeloid leukemia.
Xie, Xiaoling; Zhang, Wuju; Zhou, Xuan; Xu, Binyan; Wang, Hao; Qiu, Yingqi; Hu, Yuxing; Guo, Bin; Ye, Zhixin; Hu, Le; Zhang, Honghao; Li, Yuhua; Bai, Xiaochun.
Afiliación
  • Xie X; Department of Hematology, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China. xxl413@smu.edu.cn.
  • Zhang W; Central Laboratory, The Fifth Affiliated Hospital, Southern Medical University, 510910, Guangzhou, China.
  • Zhou X; Department of Hematology, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
  • Xu B; Department of Hematology, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
  • Wang H; Department of Hematology, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
  • Qiu Y; Department of Hematology, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
  • Hu Y; Department of Hematology, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
  • Guo B; Guangdong Provincial Key Laboratory of Bone and Joint Degenerative Diseases, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, 510515, Guangzhou, China.
  • Ye Z; Guangdong Provincial Key Laboratory of Bone and Joint Degenerative Diseases, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, 510515, Guangzhou, China.
  • Hu L; Guangdong Provincial Key Laboratory of Bone and Joint Degenerative Diseases, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, 510515, Guangzhou, China.
  • Zhang H; Department of Hematology, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China.
  • Li Y; Department of Hematology, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, China. liyuhua1974@outlook.com.
  • Bai X; Guangdong Provincial Key Laboratory of Bone and Joint Degenerative Diseases, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, 510515, Guangzhou, China. baixc15@smu.edu.cn.
Oncogene ; 42(50): 3657-3669, 2023 Dec.
Article en En | MEDLINE | ID: mdl-37872214
Conventional therapies for acute myeloid leukemia (AML) often fail to eliminate the disease-initiating leukemia stem cell (LSC) population, leading to disease relapse. Interferon-γ (IFN-γ) is a known inflammatory cytokine that promotes antitumor responses. Here, we found that low serum IFN-γ levels correlated with a higher percentage of LSCs and greater relapse incidence in AML patients. Furthermore, IFNGR1 was overexpressed in relapsed patients with AML and associated with a poor prognosis. We showed that high doses (5-10 µg/day) of IFN-γ exerted an anti-AML effect, while low doses (0.01-0.05 µg/day) of IFN-γ accelerated AML development and supported LSC self-renewal in patient-derived AML-LSCs and in an LSC-enriched MLL-AF9-driven mouse model. Importantly, targeting the IFN-γ receptor IFNGR1 by using lentiviral shRNAs or neutralizing antibodies induced AML differentiation and delayed leukemogenesis in vitro and in mice. Overall, we uncovered essential roles for IFN-γ and IFNGR1 in AML stemness and showed that targeting IFNGR1 is a strategy to decrease stemness and increase differentiation in relapsed AML patients.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Mieloide Aguda / Interferón gamma Límite: Animals / Humans Idioma: En Revista: Oncogene Asunto de la revista: BIOLOGIA MOLECULAR / NEOPLASIAS Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Mieloide Aguda / Interferón gamma Límite: Animals / Humans Idioma: En Revista: Oncogene Asunto de la revista: BIOLOGIA MOLECULAR / NEOPLASIAS Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido